The Adherens Junction-associated LIM Domain Protein Smallish Regulates Epithelial Morphogenesis

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2018 Jan 24

PMID 29358210

Citations 11

Authors

Hamze Beati

Irina Peek

Paulina Hordowska

Mona Honemann-Capito

Jade Glashauser

Fabian A Renschler

Parisa Kakanj

Andreas Ramrath

Maria Leptin

Stefan Luschnig

Silke Wiesner

Andreas Wodarz

Affiliations

Soon will be listed here.

Abstract

In epithelia, cells adhere to each other in a dynamic fashion, allowing the cells to change their shape and move along each other during morphogenesis. The regulation of adhesion occurs at the belt-shaped adherens junction, the zonula adherens (ZA). Formation of the ZA depends on components of the Par-atypical PKC (Par-aPKC) complex of polarity regulators. We have identified the Lin11, Isl-1, Mec-3 (LIM) protein Smallish (Smash), the orthologue of vertebrate LMO7, as a binding partner of Bazooka/Par-3 (Baz), a core component of the Par-aPKC complex. Smash also binds to Canoe/Afadin and the tyrosine kinase Src42A and localizes to the ZA in a planar polarized fashion. Animals lacking Smash show loss of planar cell polarity (PCP) in the embryonic epidermis and reduced cell bond tension, leading to severe defects during embryonic morphogenesis of epithelial tissues and organs. Overexpression of Smash causes apical constriction of epithelial cells. We propose that Smash is a key regulator of morphogenesis coordinating PCP and actomyosin contractility at the ZA.

Citing Articles

Actin-dependent recruitment of AGO2 to the zonula adherens.

Bridges M, Nair-Menon J, Risner A, Jimenez D, Daulagala A, Kingsley C Mol Biol Cell. 2023; 34(13):ar129.

PMID: 37819702 PMC: 10848941. DOI: 10.1091/mbc.E22-03-0099-T.

Zyxin contributes to coupling between cell junctions and contractile actomyosin networks during apical constriction.

Slabodnick M, Tintori S, Prakash M, Zhang P, Higgins C, Chen A PLoS Genet. 2023; 19(3):e1010319.

PMID: 36976799 PMC: 10081768. DOI: 10.1371/journal.pgen.1010319.

Coordination of LMO7 with FAK Signaling Sustains Epithelial Integrity in Renal Epithelia Exposed to Osmotic Pressure.

Zhen Y, Wu C, Chen H, Chang E, Jia-Jung Lee , Chen W Cells. 2022; 11(23).

PMID: 36497072 PMC: 9741450. DOI: 10.3390/cells11233805.

Lmo7 recruits myosin II heavy chain to regulate actomyosin contractility and apical domain size in Xenopus ectoderm.

Matsuda M, Chu C, Sokol S Development. 2022; 149(10).

PMID: 35451459 PMC: 9188752. DOI: 10.1242/dev.200236.

Multivalent interactions make adherens junction-cytoskeletal linkage robust during morphogenesis.

Perez-Vale K, Yow K, Johnson R, Byrnes A, Finegan T, Slep K J Cell Biol. 2021; 220(12).

PMID: 34762121 PMC: 8590279. DOI: 10.1083/jcb.202104087.

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