The Biological Changes of Umbilical Cord Mesenchymal Stem Cells in Inflammatory Environment Induced by Different Cytokines

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2018 Jan 23

PMID 29356988

Citations 19

Authors

Chao Yang

Yu Chen

Fan Li

Min You

Liwu Zhong

Wenxian Li

Bo Zhang

Qiang Chen

Affiliations

Soon will be listed here.

Abstract

Mesenchymal stem cells (MSCs) are used as therapeutic tool for the treatment of immune diseases. The inflammatory environment also influences the characteristics of MSCs after transplantation. The aim of the study was to investigate the effects of pro-inflammatory cytokines on the characteristics of umbilical cord mesenchymal stem cells (UCMSCs). UCMSCs were exposed to pro-inflammatory cytokines in vitro for 3 and 7 days, and the biological properties were analyzed. The results showed that the proliferation ability was suppressed by interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β). The adipogenic capacity was inhibited in all conditioned medium, while the chondrogenic and osteogenic capacity was enhanced by TNF-α and IL-1β in vitro. Prostaglandin E2 (PGE2) was increased by IL-1β on the third day, and angiopoietin-1 (Ang-1) was inhibited appreciably by TNF-α on the seventh day. Interleukin-6 (IL-6) was increased by TNF-α and IL-1β, and hepatocyte growth factor (HGF) was inhibited by all inflammatory cytokines. IFN-γ secretion level from human peripheral mononuclear cells (hPBMCs) was lowered by UCMSCs which had been stimulated by TNF-α or IL-1β for 3 days. Moreover, IFN-γ and TNF-α secretion level was only inhibited by UCMSCs which had been by stimulated IFN-γ for 3 days but not 7 days. Our data demonstrated that different inflammatory cytokines and the duration of treatment had different effects on the properties of UCMSCs, which might be instructive for clinical pretreatment in cellular therapeutics.

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References

Kizil C, Kyritsis N, Brand M . Effects of inflammation on stem cells: together they strive?. EMBO Rep. 2015; 16(4):416-26. PMC: 4388609. DOI: 10.15252/embr.201439702. View

Weiss M, Medicetty S, Bledsoe A, Rachakatla R, Choi M, Merchav S . Human umbilical cord matrix stem cells: preliminary characterization and effect of transplantation in a rodent model of Parkinson's disease. Stem Cells. 2005; 24(3):781-92. DOI: 10.1634/stemcells.2005-0330. View

Meisel R, Zibert A, Laryea M, Gobel U, Daubener W, Dilloo D . Human bone marrow stromal cells inhibit allogeneic T-cell responses by indoleamine 2,3-dioxygenase-mediated tryptophan degradation. Blood. 2004; 103(12):4619-21. DOI: 10.1182/blood-2003-11-3909. View

Djouad F, Charbonnier L, Bouffi C, Louis-Plence P, Bony C, Apparailly F . Mesenchymal stem cells inhibit the differentiation of dendritic cells through an interleukin-6-dependent mechanism. Stem Cells. 2007; 25(8):2025-32. DOI: 10.1634/stemcells.2006-0548. View

Jadalannagari S, Aljitawi O . Ectodermal Differentiation of Wharton's Jelly Mesenchymal Stem Cells for Tissue Engineering and Regenerative Medicine Applications. Tissue Eng Part B Rev. 2014; 21(3):314-22. DOI: 10.1089/ten.TEB.2014.0404. View

Aggarwal S, Pittenger M . Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood. 2004; 105(4):1815-22. DOI: 10.1182/blood-2004-04-1559. View

Kikuchi-Taura A, Taguchi A, Kanda T, Inoue T, Kasahara Y, Hirose H . Human umbilical cord provides a significant source of unexpanded mesenchymal stromal cells. Cytotherapy. 2012; 14(4):441-50. DOI: 10.3109/14653249.2012.658911. View

Li C, Li G, Liu M, Zhou T, Zhou H . Paracrine effect of inflammatory cytokine-activated bone marrow mesenchymal stem cells and its role in osteoblast function. J Biosci Bioeng. 2015; 121(2):213-9. DOI: 10.1016/j.jbiosc.2015.05.017. View

Wei H, Shen G, Deng X, Lou D, Sun B, Wu H . The role of IL-6 in bone marrow (BM)-derived mesenchymal stem cells (MSCs) proliferation and chondrogenesis. Cell Tissue Bank. 2013; 14(4):699-706. DOI: 10.1007/s10561-012-9354-9. View

10.

Watson N, Divers R, Kedar R, Mehindru A, Mehindru A, Borlongan M . Discarded Wharton jelly of the human umbilical cord: a viable source for mesenchymal stromal cells. Cytotherapy. 2014; 17(1):18-24. PMC: 4274214. DOI: 10.1016/j.jcyt.2014.08.009. View

11.

Ahmed M, Gaffen S . IL-17 inhibits adipogenesis in part via C/EBPα, PPARγ and Krüppel-like factors. Cytokine. 2013; 61(3):898-905. PMC: 3595389. DOI: 10.1016/j.cyto.2012.12.007. View

12.

Nagamura-Inoue T, Mukai T . Umbilical Cord is a Rich Source of Mesenchymal Stromal Cells for Cell Therapy. Curr Stem Cell Res Ther. 2015; 11(8):634-642. DOI: 10.2174/1574888x10666151026115017. View

13.

Benkhoucha M, Santiago-Raber M, Schneiter G, Chofflon M, Funakoshi H, Nakamura T . Hepatocyte growth factor inhibits CNS autoimmunity by inducing tolerogenic dendritic cells and CD25+Foxp3+ regulatory T cells. Proc Natl Acad Sci U S A. 2010; 107(14):6424-9. PMC: 2851995. DOI: 10.1073/pnas.0912437107. View

14.

Feng X, Feng G, Xing J, Shen B, Tan W, Huang D . Repeated lipopolysaccharide stimulation promotes cellular senescence in human dental pulp stem cells (DPSCs). Cell Tissue Res. 2014; 356(2):369-80. DOI: 10.1007/s00441-014-1799-7. View

15.

Scales W, Chensue S, Otterness I, Kunkel S . Regulation of monokine gene expression: prostaglandin E2 suppresses tumor necrosis factor but not interleukin-1 alpha or beta-mRNA and cell-associated bioactivity. J Leukoc Biol. 1989; 45(5):416-21. View

16.

Naaldijk Y, Johnson A, Ishak S, Meisel H, Hohaus C, Stolzing A . Migrational changes of mesenchymal stem cells in response to cytokines, growth factors, hypoxia, and aging. Exp Cell Res. 2015; 338(1):97-104. DOI: 10.1016/j.yexcr.2015.08.019. View

17.

Liu C, Xiong H, Chen K, Huang Y, Yin X . Long-term exposure to pro-inflammatory cytokines inhibits the osteogenic/dentinogenic differentiation of stem cells from the apical papilla. Int Endod J. 2015; 49(10):950-9. DOI: 10.1111/iej.12551. View

18.

Bogdan C, Nathan C . Modulation of macrophage function by transforming growth factor beta, interleukin-4, and interleukin-10. Ann N Y Acad Sci. 1993; 685:713-39. DOI: 10.1111/j.1749-6632.1993.tb35934.x. View

19.

Teshima T, Reddy P, Zeiser R . Acute Graft-versus-Host Disease: Novel Biological Insights. Biol Blood Marrow Transplant. 2015; 22(1):11-6. DOI: 10.1016/j.bbmt.2015.10.001. View

20.

Beyne-Rauzy O, Recher C, Dastugue N, Demur C, Pottier G, Laurent G . Tumor necrosis factor alpha induces senescence and chromosomal instability in human leukemic cells. Oncogene. 2004; 23(45):7507-16. DOI: 10.1038/sj.onc.1208024. View