Influence of Schistosoma Japonicum Programmed Cell Death Protein 10 on the Growth and Development of Schistosomula

Overview

Journal Parasit Vectors

Publisher Biomed Central

Specialties Parasitology
Tropical Medicine

Date 2018 Jan 20

PMID 29347959

Citations 3

Authors

Yan Ru Gao

Wen Ling Huang

Chun Lian Tang

Rong Liu

Qin Ping Zhao

Zhen Ping Ming

Hui Fen Dong

Affiliations

Soon will be listed here.

Abstract

Background: Schistosomiasis caused by Schistosoma japonicum is among the most serious endemic zoonoses in China. To study interactions between schistosomula, the pre-adult juvenile stage, and hosts, it is important to study the functions of key genes involved in schistosomula growth and development. Programmed cell death protein 10 (pcdp10) is an important apoptosis-related gene with various biological functions. This study described the molecular characterization of S. japonicum PCDP10 (SjPCDP10) and evaluated its functions in schistosomula.

Methods: Real-time quantitative polymerase chain reaction (qPCR) and western blot were used to detect Sjpcdp10 mRNA and protein levels, respectively, at different developmental stages. Immunolocalization was performed to determine SjPCDP10 expression in the parasite. RNA interference (RNAi) experiments were used to assess gene functions associated with SjPCDP10 in schistosomula growth and development.

Results: Real-time qPCR revealed that Sjpcdp10 was expressed during all investigated developmental stages and upregulated during schistosomula growth and development. Histochemical localization showed that SjPCDP10 was mainly distributed in the teguments of schistosomula in all investigated stages and part of the parenchymal area of 14-, 18-, and 21-day-old schistosomula. Following Sjpcdp10 knockdown by RNAi, the lengths, widths, areas, and volumes of schistosomula were significantly lower than those in the control group. Scanning electron microscopy showed that the body surfaces of schistosomula subjected to RNAi were seriously damaged, with few tegumental spines and sensory papillae. Transmission electron microscopy indicated that the teguments of Sjpcdp10-knockdown schistosomula were incomplete, the number of layers was reduced, and the thickness decreased significantly as compared with those in the control group. Furthermore, terminal deoxynucleotidyl transferase dUTP nick-end labelling results showed that the rate of apoptosis in Sjpcdp10-knockdown schistosomula was significantly higher than that in the control group.

Conclusions: Sjpcdp10-knockdown influenced the growth and development of schistosomula. Therefore, our results indicated that SjPCDP10 contributes to the regulation of cell apoptosis and is essential for schistosomula growth and development.

Citing Articles

Involvement of the fatty acid-binding protein in the growth of Schistosoma japonicum schistosomula.

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PMID: 34626234 DOI: 10.1007/s00436-021-07339-w.

Effects of programmed cell death protein 10 on fecundity in Schistosoma japonicum.

Gao Y, Xu J, Tang C, Cai Z, Wu Q, Xiong Y Parasitol Res. 2020; 119(4):1317-1325.

PMID: 32152713 DOI: 10.1007/s00436-020-06635-1.

Protein extract from head-foot tissue of Oncomelania hupensis promotes the growth and development of mother sporocysts of Schistosoma japonicum via upregulation of parasite aldolase gene.

Chai T, Zhu S, Dong H, Ming Z Parasitol Res. 2019; 118(6):1821-1831.

PMID: 31011809 DOI: 10.1007/s00436-019-06308-8.

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