Analysis of Helper Activity on Pokeweed Mitogen- and Interleukin 2-driven Immunoglobulin Synthesis by Neoplastic T4+ Cells

Overview

Journal J Clin Invest

Specialty General Medicine

Date 1985 Dec 1

PMID 2934407

Citations 3

Authors

F Miedema

J W van Oostveen

F G Terpstra

A W van den Wall Bake

R Willemze

E A Rauws

R Bieger

M B van t Veer

D Catovsky

C J Melief

Affiliations

Soon will be listed here.

Abstract

The neoplastic T cells of a series of seven patients with chronic T-cell neoplasia were tested for helper activity on pokeweed mitogen (PWM)-induced and interleukin 2 (IL-2)-induced Ig synthesis. The neoplastic T cells of all patients had a T3+4+8-11+I1- phenotype but differed in expression of the 3A1 antigen. The neoplastic T cells of three patients had helper activity on both PWM- and IL-2-driven Ig synthesis, and in addition produced IL-2 in response to PWM stimulation. Two of these patients had hypergammaglobulinemia. In contrast, the neoplastic T cells in the remaining four patients did not produce IL-2 and did not support PWM-driven Ig synthesis. The T4+ cells of these four patients, however, provided excellent helper activity on IL-2-driven Ig synthesis. These findings emphasize the role of IL-2 in T cell-dependent Ig synthesis and clearly show that IL-2 production is required for helper activity in the PWM-driven system. It is concluded that the combined use of PWM- and IL-2-driven Ig synthesis systems allows separate analysis of IL-2 production and T-helper activity in health and disease.

Citing Articles

Immunological abnormalities in human immunodeficiency virus (HIV)-infected asymptomatic homosexual men. HIV affects the immune system before CD4+ T helper cell depletion occurs.

Miedema F, Petit A, Terpstra F, Schattenkerk J, de Wolf F, Al B J Clin Invest. 1988; 82(6):1908-14.

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Clonal analysis of functionally distinct human CD4+ T cell subsets.

Rotteveel F, Kokkelink I, van Lier R, Kuenen B, Meager A, Miedema F J Exp Med. 1988; 168(5):1659-73.

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Evidence for a role of virulent human immunodeficiency virus (HIV) variants in the pathogenesis of acquired immunodeficiency syndrome: studies on sequential HIV isolates.

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References

Sauerwein R, van der Meer W, Drager A, Aarden L . Interleukin 2 induces T cell-dependent IgM production in human B cells. Eur J Immunol. 1985; 15(6):611-6. DOI: 10.1002/eji.1830150615. View

Miedema F, van Oostveen J, Sauerwein R, Terpstra F, Aarden L, Melief C . Induction of immunoglobulin synthesis by interleukin 2 is T4+/T8- cell dependent. A role for interleukin 2 in the pokeweed mitogen-driven system. Eur J Immunol. 1985; 15(2):107-12. DOI: 10.1002/eji.1830150202. View

Broder S, Edelson R, LUTZNER M, Nelson D, Macdermott R, Durm M . The Sézary syndrome: a malignant proliferation of helper T cells. J Clin Invest. 1976; 58(6):1297-306. PMC: 333301. DOI: 10.1172/JCI108585. View

Siegal F, Siegal M . Enhancement by irradiated T cells of human plasma cell production: dissection of helper and suppressor functions in vitro. J Immunol. 1977; 118(2):642-7. View

van Oers M, Zeijlemaker W, Schellekens P . Separation and properties of EA-rosette-forming lymphocytes in humans. Eur J Immunol. 1977; 7(3):143-50. DOI: 10.1002/eji.1830070306. View

Broder S, Uchiyama T, Waldmann T . Neoplasms of immunoregulatory cells. Am J Clin Pathol. 1979; 72(4 Suppl):724-31. View

Reinherz E, Kung P, Pesando J, Ritz J, Goldstein G, Schlossman S . Ia determinants on human T-cell subsets defined by monoclonal antibody. Activation stimuli required for expression. J Exp Med. 1979; 150(6):1472-82. PMC: 2185738. DOI: 10.1084/jem.150.6.1472. View

Reinherz E, Schlossman S . The differentiation and function of human T lymphocytes. Cell. 1980; 19(4):821-7. DOI: 10.1016/0092-8674(80)90072-0. View

Haynes B, Bunn P, Mann D, Thomas C, Eisenbarth G, Minna J . Cell surface differentiation antigens of the malignant T cell in Sezary syndrome and mycosis fungoides. J Clin Invest. 1981; 67(2):523-30. PMC: 370595. DOI: 10.1172/JCI110062. View

10.

Foon K, Naiem F, Saxon A, Stevens R, Gale R . Leukemia of T-helper lymphocytes: clinical and functional features. Leuk Res. 1981; 5(1):1-10. DOI: 10.1016/0145-2126(81)90091-6. View

11.

Thomas Y, Rogozinski L, Irigoyen O, Shen H, Talle M, Goldstein G . Functional analysis of human T cell subsets defined by monoclonal antibodies. V. Suppressor cells within the activated OKT4+ population belong to a distinct subset. J Immunol. 1982; 128(3):1386-90. View

12.

Gramatzki M, DOLAN M, Fauci A, Maples J, Bonnard G, Strong D . Immunologic characterization of a helper T-cell lymphoma. Blood. 1982; 59(4):702-8. View

13.

Rumke H, Miedema F, ten Berge I, Terpstra F, van der Reijden H, van de Griend R . Functional properties of T cells in patients with chronic T gamma lymphocytosis and chronic T cell neoplasia. J Immunol. 1982; 129(1):419-26. View

14.

Vyth-Dreese F, van der Reijden H, De Vries J . Phorbol-ester-mediated induction and augmentation of mitogenesis and interleukin-2 production in human T-cell lymphoproliferative disease. Blood. 1982; 60(6):1437-46. View

15.

Yamada Y . Phenotypic and functional analysis of leukemic cells from 16 patients with adult T-cell leukemia/lymphoma. Blood. 1983; 61(1):192-9. View

16.

Catovsky D, Wechsler A, Matutes E, Gomez R, Bourikas G, Cherchi M . The membrane phenotype of T-prolymphocytic leukaemia. Scand J Haematol. 1982; 29(5):398-404. DOI: 10.1111/j.1600-0609.1982.tb00614.x. View

17.

Lamb J, Zanders E, Feldmann M, Lake P, Eckels D, Woody J . The dissociation of interleukin-2 production and antigen-specific helper activity by clonal analysis. Immunology. 1983; 50(3):397-405. PMC: 1454249. View

18.

Inaba K, Granelli-Piperno A, Steinman R . Dendritic cells induce T lymphocytes to release B cell-stimulating factors by an interleukin 2-dependent mechanism. J Exp Med. 1983; 158(6):2040-57. PMC: 2187156. DOI: 10.1084/jem.158.6.2040. View

19.

Miedema F, Terpstra F, Smit J, Daenen S, Gerrits W, Hegde U . Functional properties of neoplastic T cells in adult T cell lymphoma/leukemia patients from the Caribbean. Blood. 1984; 63(2):477-81. View

20.

Waldmann T, Greene W, SARIN P, Saxinger C, Blayney D, Blattner W . Functional and phenotypic comparison of human T cell leukemia/lymphoma virus positive adult T cell leukemia with human T cell leukemia/lymphoma virus negative Sézary leukemia, and their distinction using anti-Tac. Monoclonal antibody identifying the.... J Clin Invest. 1984; 73(6):1711-8. PMC: 437083. DOI: 10.1172/JCI111379. View