Isolation and Characterization of Key Genes That Promote Flavonoid Accumulation in Purple-leaf Tea (Camellia Sinensis L.)

Overview

Journal Sci Rep

Specialty Science

Date 2018 Jan 11

PMID 29317677

Citations 30

Authors

Xiujuan He

Xuecheng Zhao

Liping Gao

Xingxing Shi

Xinlong Dai

Yajun Liu

Tao Xia

Yunsheng Wang

Affiliations

Soon will be listed here.

Abstract

There were several high concentrations of flavonoid components in tea leaves that present health benefits. A novel purple-leaf tea variety, 'Mooma1', was obtained from the natural hybrid population of Longjing 43 variety. The buds and young leaves of 'Mooma1' were displayed in bright red. HPLC and LC-MS analysis showed that anthocyanins and O-Glycosylated flavonols were remarkably accumulated in the leaves of 'Mooma1', while the total amount of catechins in purple-leaf leaves was slightly decreased compared with the control. A R2R3-MYB transcription factor (CsMYB6A) and a novel UGT gene (CsUGT72AM1), that were highly expressed in purple leaf were isolated and identified by transcriptome sequencing. The over-expression of transgenic tobacco confirmed that CsMYB6A can activate the expression of flavonoid-related structural genes, especially CHS and 3GT, controlling the accumulation of anthocyanins in the leaf of transgenic tobacco. Enzymatic assays in vitro confirmed that CsUGT72AM1 has catalytic activity as a flavonol 3-O-glucosyltransferase, and displayed broad substrate specificity. The results were useful for further elucidating the molecular mechanisms of the flavonoid metabolic fluxes in the tea plant.

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