Protein Profile in Aspergillus Nidulans Recombinant Strains Overproducing Heterologous Enzymes

Overview

Journal Microb Biotechnol

Specialties Biotechnology
Microbiology

Date 2018 Jan 10

PMID 29316319

Citations 5

Authors

Mariane Paludetti Zubieta

Fabiano Jares Contesini

Marcelo Ventura Rubio

Any Elisa de Souza Schmidt Goncalves

Jaqueline Aline Gerhardt

Rolf Alexander Prade

Andre Ricardo de Lima Damasio

Affiliations

Soon will be listed here.

Abstract

Filamentous fungi are robust cell factories and have been used for the production of large quantities of industrially relevant enzymes. However, the production levels of heterologous proteins still need to be improved. Therefore, this article aimed to investigate the global proteome profiling of Aspergillus nidulans recombinant strains in order to understand the bottlenecks of heterologous enzymes production. About 250, 441 and 424 intracellular proteins were identified in the control strain Anid_pEXPYR and in the recombinant strains Anid_AbfA and Anid_Cbhl respectively. In this context, the most enriched processes in recombinant strains were energy pathway, amino acid metabolism, ribosome biogenesis, translation, endoplasmic reticulum and oxidative stress, and repression under secretion stress (RESS). The global protein profile of the recombinant strains Anid_AbfA and Anid_Cbhl was similar, although the latter strain secreted more recombinant enzyme than the former. These findings provide insights into the bottlenecks involved in the secretion of recombinant proteins in A. nidulans, as well as in regard to the rational manipulation of target genes for engineering fungal strains as microbial cell factories.

Citing Articles

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Protein profile in Aspergillus nidulans recombinant strains overproducing heterologous enzymes.

Zubieta M, Contesini F, Rubio M, Goncalves A, Gerhardt J, Prade R Microb Biotechnol. 2018; 11(2):346-358.

PMID: 29316319 PMC: 5812239. DOI: 10.1111/1751-7915.13027.

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