Advantages of Amplifluor-like SNP Markers over KASP in Plant Genotyping

Overview

Journal BMC Plant Biol

Publisher Biomed Central

Specialty Biology

Date 2018 Jan 4

PMID 29297326

Citations 18

Authors

Satyvaldy Jatayev

Akhylbek Kurishbayev

Lyudmila Zotova

Gulmira Khasanova

Dauren Serikbay

Askar Zhubatkanov

Makpal Botayeva

Aibek Zhumalin

Arysgul Turbekova

Kathleen Soole

Peter Langridge

Yuri Shavrukov

Affiliations

Soon will be listed here.

Abstract

Background: KASP (KBioscience Competitive Allele Specific PCR) and Amplifluor (Amplification with fluorescence) SNP markers are two prominent technologies based upon a shared identical Allele-specific PCR platform.

Methods: Amplifluor-like SNP and KASP analysis was carried out using published and own design of Universal probes (UPs) and Gene-specific primers (GSPs).

Results: Advantages of the Amplifluor-like system over KASP include the significantly lower costs and much greater flexibility in the adjustment and development of 'self-designed' dual fluorescently-labelled UPs and regular GSPs. The presented results include optimisation of 'tail' length in UPs and GSPs, protocol adjustment, and the use of various fluorophores in different qPCR instruments. The compatibility of the KASP Master-mix in both original and Amplifluor-like systems has been demonstrated in the presented results, proving their similar principles. Results of SNP scoring with rare alleles in addition to more frequently occurring alleles are shown.

Conclusions: The Amplifluor-like system produces SNP genotyping results with a level of sensitivity and accuracy comparable to KASP but at a significantly cheaper cost and with much greater flexibility for UPs with self-designed GSPs.

Citing Articles

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