Evaluation of Genetic Diversity Among Olive Trees ( L.) from Jordan

Overview

Journal Front Plant Sci

Date 2024 Aug 21

PMID 39166249

Authors

Mazen A Al-Kilani

Francesca Taranto

Nunzio DAgostino

Cinzia Montemurro

Angjelina Belaj

Salam Ayoub

Randa Albdaiwi

Shireen Hasan

Ayed M Al-Abdallat

Affiliations

Soon will be listed here.

Abstract

This study aimed to identify and evaluate the genetic diversity of olive trees in Jordan, a country located in the eastern Mediterranean, where olive domestication originated. For this purpose, a total of 386 olive trees were analyzed, including 338 collected from two surveys (JOCC-1 and JOCC-2) across seven regions, and 48 selected accessions from the Olive Germplasm Bank of Jordan (JGBOC). These trees underwent comprehensive phenotypic and molecular characterization using different tools. Significant differences in morphological traits were detected among tested regions using the -square test. Principal components analysis revealed that fruit color change and growth habit as the most discriminating traits, segregating the trees into two groups, with the first group including the Kanabisi cultivar and the second group including the Kfari Baladi cultivar. Utilizing Kompetitive Allele Specific PCR assay, two sets of informative SNPs were used for the genetic diversity analysis. Cladograms were constructed using the maximum likelihood method, revealing a consistent pattern where two clades containing identical genotypes were observed to cluster with the Kfari Baladi or Kanabisi. In addition, the SNP data was used to perform a comparative analysis with the Worldwide Olive Germplasm Bank of Córdoba, which revealed 73 unreported olive genotypes from Jordan. Genetic structure analyses using Discriminant Analysis of Principal Components (DAPC) identified four clusters with distinctive patterns of relatedness among 149 unique accessions, including 52 olive accessions from various Mediterranean countries (IOCC-3). ADMIXTURE analysis revealed four genetic clusters, consistent with the clustering observed in DAPC and cladogram analysis, indicating a high level of genetic admixture among Jordanian olive germplasm. In conclusion, the results show that olive trees in Jordan are highly diverse, providing valuable information for future conservation and management plans.

References

Julca I, Marcet-Houben M, Cruz F, Gomez-Garrido J, Gaut B, Diez C . Genomic evidence for recurrent genetic admixture during the domestication of Mediterranean olive trees (Olea europaea L.). BMC Biol. 2020; 18(1):148. PMC: 7586694. DOI: 10.1186/s12915-020-00881-6. View

Ben Ayed R, Rebai A . Tunisian Table Olive Oil Traceability and Quality Using SNP Genotyping and Bioinformatics Tools. Biomed Res Int. 2019; 2019:8291341. PMC: 6381586. DOI: 10.1155/2019/8291341. View

Diez C, Trujillo I, Martinez-Urdiroz N, Barranco D, Rallo L, Marfil P . Olive domestication and diversification in the Mediterranean Basin. New Phytol. 2014; 206(1):436-447. DOI: 10.1111/nph.13181. View

Mousavi S, Mariotti R, Bagnoli F, Costantini L, Cultrera N, Arzani K . The eastern part of the Fertile Crescent concealed an unexpected route of olive (Olea europaea L.) differentiation. Ann Bot. 2017; 119(8):1305-1318. PMC: 5604562. DOI: 10.1093/aob/mcx027. View

Baldoni L, Tosti N, Ricciolini C, Belaj A, Arcioni S, Pannelli G . Genetic structure of wild and cultivated olives in the central Mediterranean basin. Ann Bot. 2006; 98(5):935-42. PMC: 2803593. DOI: 10.1093/aob/mcl178. View

Winfield M, Burridge A, Ordidge M, Harper H, Wilkinson P, Thorogood D . Development of a minimal KASP marker panel for distinguishing genotypes in apple collections. PLoS One. 2020; 15(11):e0242940. PMC: 7703965. DOI: 10.1371/journal.pone.0242940. View

Jimenez-Ruiz J, Ramirez-Tejero J, Fernandez-Pozo N, Leyva-Perez M, Yan H, de la Rosa R . Transposon activation is a major driver in the genome evolution of cultivated olive trees (Olea europaea L.). Plant Genome. 2020; 13(1):e20010. DOI: 10.1002/tpg2.20010. View

Jombart T, Ahmed I . adegenet 1.3-1: new tools for the analysis of genome-wide SNP data. Bioinformatics. 2011; 27(21):3070-1. PMC: 3198581. DOI: 10.1093/bioinformatics/btr521. View

Sales H, Satovic Z, Alves M, Fevereiro P, Nunes J, Vaz Patto M . Accessing Ancestral Origin and Diversity Evolution by Net Divergence of an Ongoing Domestication Mediterranean Olive Tree Variety. Front Plant Sci. 2021; 12:688214. PMC: 8265600. DOI: 10.3389/fpls.2021.688214. View

10.

Kaniewski D, Marriner N, Morhange C, Khater C, Terral J, Besnard G . Climate change threatens olive oil production in the Levant. Nat Plants. 2023; 9(2):219-227. DOI: 10.1038/s41477-022-01339-z. View

11.

El Bakkali A, Essalouh L, Tollon C, Rivallan R, Mournet P, Moukhli A . Characterization of Worldwide Olive Germplasm Banks of Marrakech (Morocco) and Córdoba (Spain): Towards management and use of olive germplasm in breeding programs. PLoS One. 2019; 14(10):e0223716. PMC: 6797134. DOI: 10.1371/journal.pone.0223716. View

12.

Gros-Balthazard M, Besnard G, Sarah G, Holtz Y, Leclercq J, Santoni S . Evolutionary transcriptomics reveals the origins of olives and the genomic changes associated with their domestication. Plant J. 2019; 100(1):143-157. PMC: 6851578. DOI: 10.1111/tpj.14435. View

13.

Mariotti R, Belaj A, de la Rosa R, Muleo R, Cirilli M, Forgione I . Genealogical tracing of Olea europaea species and pedigree relationships of var. europaea using chloroplast and nuclear markers. BMC Plant Biol. 2023; 23(1):452. PMC: 10521521. DOI: 10.1186/s12870-023-04440-3. View

14.

Diez C, Trujillo I, Barrio E, Belaj A, Barranco D, Rallo L . Centennial olive trees as a reservoir of genetic diversity. Ann Bot. 2011; 108(5):797-807. PMC: 3177672. DOI: 10.1093/aob/mcr194. View

15.

Kaya H, Akdemir D, Lozano R, Cetin O, Sozer Kaya H, Sahin M . Genome wide association study of 5 agronomic traits in olive (Olea europaea L.). Sci Rep. 2019; 9(1):18764. PMC: 6904458. DOI: 10.1038/s41598-019-55338-w. View

16.

Arenas-Castro S, Goncalves J, Moreno M, Villar R . Projected climate changes are expected to decrease the suitability and production of olive varieties in southern Spain. Sci Total Environ. 2020; 709:136161. DOI: 10.1016/j.scitotenv.2019.136161. View

17.

Belaj A, Ninot A, Gomez-Galvez F, El Riachy M, Gurbuz-Veral M, Torres M . Utility of EST-SNP Markers for Improving Management and Use of Olive Genetic Resources: A Case Study at the Worldwide Olive Germplasm Bank of Córdoba. Plants (Basel). 2022; 11(7). PMC: 9002360. DOI: 10.3390/plants11070921. View

18.

Mariotti R, Belaj A, de la Rosa R, Leon L, Brizioli F, Baldoni L . EST-SNP Study of L. Uncovers Functional Polymorphisms between Cultivated and Wild Olives. Genes (Basel). 2020; 11(8). PMC: 7465833. DOI: 10.3390/genes11080916. View

19.

Goonetilleke S, March T, Wirthensohn M, Arus P, Walker A, Mather D . Genotyping by Sequencing in Almond: SNP Discovery, Linkage Mapping, and Marker Design. G3 (Bethesda). 2017; 8(1):161-172. PMC: 5765344. DOI: 10.1534/g3.117.300376. View

20.

Slobodova N, Sharko F, Gladysheva-Azgari M, Petrova K, Tsiupka S, Tsiupka V . Genetic Diversity of Common Olive ( L.) Cultivars from Nikita Botanical Gardens Collection Revealed Using RAD-Seq Method. Genes (Basel). 2023; 14(7). PMC: 10379327. DOI: 10.3390/genes14071323. View