Repetitive Maturation of Oocytes From Non-Stimulated Xenografted Ovarian Tissue From a Prepubertal Patient Indicating the Independence of Human Ovarian Tissue

Overview

Journal Geburtshilfe Frauenheilkd

Specialty Gynecology & Obstetrics

Date 2017 Dec 23

PMID 29269958

Citations 6

Authors

Nathalie Raffel

Laura Lotz

Inge Hoffmann

Jana Liebenthron

Stephan Soder

Matthias W Beckmann

Ralf Dittrich

Affiliations

Soon will be listed here.

Abstract

Introduction: Modern anti-cancer strategies have distinctly increased survival rates; nevertheless, often accompanied by sterility. Currently, the only option for preserving fertility in prepubertal females is to cryopreserve ovarian tissue and re-transplant frozen-thawed tissue to restore fertility after treatment. Our aim was to report the occurrence of repetitive antral follicle formation and oocyte maturation in a prepubescent ovarian tissue xenograft without exogenous hormone stimulation.

Material And Methods: Frozen-thawed ovarian tissue from a 6-year-old patient suffering from nephroblastoma was xenotransplanted in oophorectomized severe combined immunodeficiency (SCID) mice to evaluate follicle development.

Ergebnisse: Repetitive follicle development to the antral stage occurred in the same xenograft of prepubertal ovarian tissue without exogenous hormone administration; 37 days after retrieving a maturing oocyte (this first retrieval has been previously published), another, completely mature oocyte was harvested from the xenograft. Subsequent histological evaluation of the grafted tissue showed primordial follicles, nearly all stages of developing follicles, as well as large atretic ones. Many clusters with dormant primordial follicles were also present.

Conclusion: Xenotransplanted prepubertal ovarian tissue has the potential for repetitive oocyte retrieval cycles without administering exogenous hormones. The results indicate that the human ovarian tissue might be able to synchronize the hypothalamus-hypophysis-axes of the mouse to the physiological human cycle; this should be investigated in future studies.

Citing Articles

A Systematic Review of Ovarian Tissue Transplantation Outcomes by Ovarian Tissue Processing Size for Cryopreservation.

Diaz A, Kubo H, Handa N, Hanna M, Laronda M Front Endocrinol (Lausanne). 2022; 13:918899.

PMID: 35774145 PMC: 9239173. DOI: 10.3389/fendo.2022.918899.

Effect of Previous Alkylating Agent Exposure on Follicle Numbers in Cryopreserved Prepubertal and Young Adult Ovarian Tissue after Long-Term Xenografting.

Nurmio M, Asadi-Azarbaijani B, Hou M, Kivio R, Toppari J, Tinkanen H Cancers (Basel). 2022; 14(2).

PMID: 35053561 PMC: 8773859. DOI: 10.3390/cancers14020399.

Hormonal response in patients transplanted with cryopreserved ovarian tissue is independent of whether freezing was performed in childhood or adulthood.

Hornshoj V, Dueholm M, Mamsen L, Ernst E, Andersen C J Assist Reprod Genet. 2021; 38(11):3039-3045.

PMID: 34617199 PMC: 8608935. DOI: 10.1007/s10815-021-02320-z.

Pediatric oncofertility: an update.

Lau G, Schaeffer A Transl Androl Urol. 2020; 9(5):2416-2421.

PMID: 33209715 PMC: 7658128. DOI: 10.21037/tau-20-991.

Hormonal Stimulation of Human Ovarian Xenografts in Mice: Studying Folliculogenesis, Activation, and Oocyte Maturation.

Wall M, Padmanabhan V, Shikanov A Endocrinology. 2020; 161(12).

PMID: 33099627 PMC: 7671278. DOI: 10.1210/endocr/bqaa194.

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