The B-Box Domain Protein BBX21 Promotes Photomorphogenesis

Overview

Journal Plant Physiol

Specialty Physiology

Date 2017 Dec 21

PMID 29259103

Citations 53

Authors

Dongqing Xu

Yan Jiang

Jian Li

Magnus Holm

Xing Wang Deng

Affiliations

Soon will be listed here.

Abstract

B-box-containing (BBX) proteins play critical roles in a variety of cellular and developmental processes in plants. BBX21 (also known as SALT TOLERANCE HOMOLOG2), which contains two B-box domains in tandem at the N terminus, has been previously demonstrated as a key component involved in the COP1-HY5 signaling hub. However, the exact molecular and physiological roles of B-box domains in BBX21 are largely unclear. Here, we found that structurally disruption of the second B-box domain, but not the first one, in BBX21 completely abolishes its biological and physiological activity in conferring hyperphotomorphogenetic phenotype in Arabidopsis (). Intact B-box domains in BBX21 are not required for interaction with COP1 and its degradation by COP1 via the 26S proteasome system. However, disruption of the second B-box of BBX21 nearly impairs its ability for binding of within the promoter both in vitro and in vivo, as well as controlling and HY5-regulated gene expression in Arabidopsis seedlings. Taken together, this study provides a mechanistic framework in which BBX21 directly binds to the present in the promoter possibly through its second B-box domain, which in turn controls and HY5-regulated gene expression to promote photomorphogenesis.

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