The NcZrg-17 Gene of Neurospora Crassa Encodes a Cation Diffusion Facilitator Transporter Required for Vegetative Development, Tolerance to Endoplasmic Reticulum Stress and Cellulose Degradation Under Low Zinc Conditions

Overview

Journal Curr Genet

Specialty Genetics

Date 2017 Dec 20

PMID 29256005

Citations 4

Authors

Anand Tiwari

Serena Daniel Ngiilmei

Ranjan Tamuli

Affiliations

Soon will be listed here.

Abstract

The Neurospora crassa gene NcZrg-17 encodes a membrane protein with homology to the cation diffusion facilitator (CDF) family of transporters. We analyzed the phenotypic and functional characteristics of ΔNcZrg-17 and the implications of these characteristics in vivo. The ΔNcZrg-17 mutant showed several phenotypes that are zinc suppressible such as reduced growth rate, short aerial hyphae, increased hyphal branching, early and enhanced conidiation and delayed conidial germination. Furthermore, the NcZrg-17 gene was found to be crucial for survival in the presence of endoplasmic reticulum (ER) stress inducing chemical agents. In addition, we found that ΔNcZrg-17 mutant is defective in protein secretion on cellulose media under low zinc conditions, pointing towards a physiological role for NcZrg-17 in N. crassa. A gradual and delayed transcriptional upregulation (~ threefold) of NcZrg-17 on exposure to low zinc suggests its role in adaptation to low zinc rather than zinc homeostasis. Together our findings support a function of NcZrg-17 in normal vegetative growth, tolerance to ER stress and degradation of cellulose under low zinc conditions in N. crassa.

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