Characterization of Ctr Family Genes and the Elucidation of Their Role in the Life Cycle of Neurospora Crassa

Overview

Journal Fungal Genet Biol

Specialties Biology
Genetics
Microbiology

Date 2009 Dec 26

PMID 20034585

Citations 10

Authors

Premsagar Korripally

Anand Tiwari

Adhikarla Haritha

Patnala Kiranmayi

Manjula Bhanoori

Affiliations

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Abstract

Transcriptional analysis using qRT-PCR of 62 metal ion transporters during conidial germination of Neurospora crassa showed a significant up regulation of a hypothetical copper transporter gene, tcu-1, that belongs to the Ctr family. Herein we characterised the Ctr family genes (tcu-1, tcu-2 and tcu-3) and deciphered their role in various developmental phases of the N. crassa life cycle. Cross complementation assays in copper uptake mutant of Saccharomyces cerevisiae revealed that tcu-1, tcu-2 and tcu-3 are functional homologs of S. cerevisiae copper transporters. Expression studies of Ctr family members in various developmental phases of N. crassa showed differential expression pattern for high-affinity copper transporter, TCU1. Functional analysis of their gene knockout mutants showed that tcu-1 is essential for saprophytic conidial germination, vegetative growth and perithecia development under copper limited conditions while conidiation remained unaffected.

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