Labeling and Identifying Cell-specific Proteomes in the Mouse Brain

Overview

Journal Nat Biotechnol

Specialty Biotechnology

Date 2017 Dec 19

PMID 29251727

Citations 45

Authors

Toke P Krogager

Russell J Ernst

Thomas S Elliott

Laura Calo

Vaclav Beranek

Ernesto Ciabatti

Maria Grazia Spillantini

Marco Tripodi

Michael H Hastings

Jason W Chin

Affiliations

Soon will be listed here.

Abstract

We develop an approach to tag proteomes synthesized by specific cell types in dissociated cortex, brain slices, and the brains of live mice. By viral-mediated expression of an orthogonal pyrrolysyl-tRNA synthetase-tRNAXXX pair in a cell type of interest and providing a non-canonical amino acid with a chemical handle, we selectively label neuronal or glial proteomes. The method enables the identification of proteins from spatially and genetically defined regions of the brain.

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