Co-infection of Sexually Transmitted Pathogens and Human Papillomavirus in Cervical Samples of Women of Brazil

Overview

Journal BMC Infect Dis

Publisher Biomed Central

Specialty Infectious Diseases

Date 2017 Dec 17

PMID 29246195

Citations 13

Authors

Aline Teixeira Amorim

Lucas Miranda Marques

Guilherme Barreto Campos

Tassia Neves Lobao

Vanesca de Souza Lino

Ricardo Cesar Cintra

Maria Antonieta Andreoli

Luisa Lina Villa

Enrique Boccardo

Antonio Carlos Ricardo Braga Junior

Rossana Veronica Mendoza Lopez

Djanilson Barbosa Dos Santos

Gerson Maciel de Souza

Carla Cristina Romano

Jorge Timenetsky

Affiliations

Soon will be listed here.

Abstract

Background: Some sexually transmitted infectious agents, such as Chlamydia trachomatis and Herpes simplex, cause local inflammation, and could contribute to Human Papillomavirus (HPV) and cervical lesion progression. Thus, the aim of this study was to determine any association between the presence of microorganisms of gynecological importance, sexual behavior, clinical and demographical variables to the development and progress of cervical lesions.

Methods: One hundred and thirty-two women between 14 and 78 years and living at Vitória da Conquista, Bahia, Brazil, were included (62 individuals with cervical lesions and 70 without lesions). They answered a questionnaire to provide data for a socioeconomic and sexual activity profile. Samples of cervical swabs were collected and analyzed by PCR to detect genital microorganisms and HPV. Quantitative PCR was used to detect and quantify Ureaplasma urealyticum and Ureaplasma parvum. Univariate and multiple logistic regression were performed to measure the association with the cervical lesions, and an odds ratio (OR) with 95% confidence intervals (95%CI) were calculated. The Mann-Whitney U test was also used to compare the microorganism load in the case and control groups. The significance level was 5% in all hypotheses tested.

Results: Cervical lesions were associated with: women in a stable sexual relationship (OR = 14.21, 95%CI = 3.67-55.018), positive PCR for HPV (OR = 16.81, 95%CI = 4.19-67.42), Trichomonas vaginalis (OR = 8.566, 95%CI = 2.04-35.94) and Gardnerella vaginalis (OR = 6.13, 95%CI = 1.53-24.61), adjusted by age and qPCR for U. parvum. U. parvum load showed a statistical difference between the case and control groups (p-value = 0.002).

Conclusion: Variables such as stable relationship, HPV, T. vaginalis, G. vaginalis were associated with cervical lesions in epidemiological studies. U. parvum load was higher in woman with cervical lesions compared with women without lesions. Additional studies are needed to better understand the role of these factors in cervical lesion development.

Citing Articles

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Trichomonas vaginalis infection and risk of cervical neoplasia: A systematic review and meta-analysis.

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Bioinformatic Analyzes of the Association Between Upregulated Expression of Gene -Induced Gene Mutation and Prognosis of Cervical Cancer.

Chen H, Zhao L, Liu J, Zhou H, Wang X, Fang X Front Med (Lausanne). 2022; 9:815450.

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