Heat-killed Lactobacillus Casei Confers Broad Protection Against Influenza A Virus Primary Infection and Develops Heterosubtypic Immunity Against Future Secondary Infection

Overview

Journal Sci Rep

Specialty Science

Date 2017 Dec 14

PMID 29234060

Citations 59

Authors

Yu-Jin Jung

Young-Tae Lee

Vu Le Ngo

Young-Hee Cho

Eun-Ju Ko

Sung-Moon Hong

Ki-Hye Kim

Ji-Hun Jang

Joon-Suk Oh

Min-Kyung Park

Cheol-Hyun Kim

Jun Sun

Sang-Moo Kang

Affiliations

Soon will be listed here.

Abstract

Lactic acid bacteria (LAB) are the common probiotics. Here, we investigated the antiviral protective effects of heat-killed LAB strain Lactobacillus casei DK128 (DK128) on influenza viruses. Intranasal treatment of mice with DK128 conferred protection against different subtypes of influenza viruses by lessening weight loss and lowering viral loads. Protection via heat-killed DK128 was correlated with an increase in alveolar macrophage cells in the lungs and airways, early induction of virus specific antibodies, reduced levels of pro-inflammatory cytokines and innate immune cells. Importantly, the mice that were protected against primary viral infection as a result of heat-killed DK128 pretreatment developed subsequent heterosubtypic immunity against secondary virus infection. For protection against influenza virus via heat-killed DK128 pretreatment, B cells and partially CD4 T cells but not CD8 T cells were required as inferred from studies using knockout mouse models. Our study provides insight into how hosts can be equipped with innate and adaptive immunity via heat-killed DK128 treatment to protect against influenza virus, supporting that heat-killed LAB may be developed as anti-virus probiotics.

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References

Kishino S, Ogawa J, Yokozeki K, Shimizu S . Metabolic diversity in biohydrogenation of polyunsaturated fatty acids by lactic acid bacteria involving conjugated fatty acid production. Appl Microbiol Biotechnol. 2009; 84(1):87-97. DOI: 10.1007/s00253-009-1949-0. View

Nakayama Y, Moriya T, Sakai F, Ikeda N, Shiozaki T, Hosoya T . Oral administration of Lactobacillus gasseri SBT2055 is effective for preventing influenza in mice. Sci Rep. 2014; 4:4638. PMC: 3982165. DOI: 10.1038/srep04638. View

Youn H, Lee Y, Lee D, Park J, Yuk S, Lee H . Effect of intranasal administration of Lactobacillus fermentum CJL-112 on horizontal transmission of influenza virus in chickens. Poult Sci. 2012; 91(10):2517-22. DOI: 10.3382/ps.2012-02334. View

Yeo J, Lee H, Kim J, Lee J, Park S, Choi I . Lactobacillus fermentum CJL-112 protects mice against influenza virus infection by activating T-helper 1 and eliciting a protective immune response. Int Immunopharmacol. 2013; 18(1):50-4. DOI: 10.1016/j.intimp.2013.10.020. View

Racedo S, Villena J, Medina M, Aguero G, Rodriguez V, Alvarez S . Lactobacillus casei administration reduces lung injuries in a Streptococcus pneumoniae infection in mice. Microbes Infect. 2006; 8(9-10):2359-66. DOI: 10.1016/j.micinf.2006.04.022. View

Park M, Ngo V, Kwon Y, Lee Y, Yoo S, Cho Y . Lactobacillus plantarum DK119 as a probiotic confers protection against influenza virus by modulating innate immunity. PLoS One. 2013; 8(10):e75368. PMC: 3790790. DOI: 10.1371/journal.pone.0075368. View

Hori T, Kiyoshima J, Shida K, Yasui H . Effect of intranasal administration of Lactobacillus casei Shirota on influenza virus infection of upper respiratory tract in mice. Clin Diagn Lab Immunol. 2001; 8(3):593-7. PMC: 96107. DOI: 10.1128/CDLI.8.3.593-597.2001. View

Kikuchi Y, Kunitoh-Asari A, Hayakawa K, Imai S, Kasuya K, Abe K . Oral administration of Lactobacillus plantarum strain AYA enhances IgA secretion and provides survival protection against influenza virus infection in mice. PLoS One. 2014; 9(1):e86416. PMC: 3899257. DOI: 10.1371/journal.pone.0086416. View

Tong S, Zhu X, Li Y, Shi M, Zhang J, Bourgeois M . New world bats harbor diverse influenza A viruses. PLoS Pathog. 2013; 9(10):e1003657. PMC: 3794996. DOI: 10.1371/journal.ppat.1003657. View

10.

Yasui H, Kiyoshima J, Hori T . Reduction of influenza virus titer and protection against influenza virus infection in infant mice fed Lactobacillus casei Shirota. Clin Diagn Lab Immunol. 2004; 11(4):675-9. PMC: 440622. DOI: 10.1128/CDLI.11.4.675-679.2004. View

11.

Tate M, Schilter H, Brooks A, Reading P . Responses of mouse airway epithelial cells and alveolar macrophages to virulent and avirulent strains of influenza A virus. Viral Immunol. 2011; 24(2):77-88. DOI: 10.1089/vim.2010.0118. View

12.

Parvez S, Malik K, Ah Kang S, Kim H . Probiotics and their fermented food products are beneficial for health. J Appl Microbiol. 2006; 100(6):1171-85. DOI: 10.1111/j.1365-2672.2006.02963.x. View

13.

Kobayashi N, Saito T, Uematsu T, Kishi K, Toba M, Kohda N . Oral administration of heat-killed Lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice. Int Immunopharmacol. 2010; 11(2):199-203. DOI: 10.1016/j.intimp.2010.11.019. View

14.

Hosoi T, Hirose R, Saegusa S, Ametani A, Kiuchi K, Kaminogawa S . Cytokine responses of human intestinal epithelial-like Caco-2 cells to the nonpathogenic bacterium Bacillus subtilis (natto). Int J Food Microbiol. 2003; 82(3):255-64. DOI: 10.1016/s0168-1605(02)00311-2. View

15.

Fraser C, Donnelly C, Cauchemez S, Hanage W, Van Kerkhove M, Hollingsworth T . Pandemic potential of a strain of influenza A (H1N1): early findings. Science. 2009; 324(5934):1557-61. PMC: 3735127. DOI: 10.1126/science.1176062. View

16.

Lee Y, Lee Y, Kim M, Gewirtz A, Kang S . A Novel Vaccination Strategy Mediating the Induction of Lung-Resident Memory CD8 T Cells Confers Heterosubtypic Immunity against Future Pandemic Influenza Virus. J Immunol. 2016; 196(6):2637-45. PMC: 4779729. DOI: 10.4049/jimmunol.1501637. View

17.

Hanniffy S, Wiedermann U, Repa A, Mercenier A, Daniel C, Fioramonti J . Potential and opportunities for use of recombinant lactic acid bacteria in human health. Adv Appl Microbiol. 2004; 56:1-64. DOI: 10.1016/S0065-2164(04)56001-X. View

18.

Hori T, Kiyoshima J, Shida K, Yasui H . Augmentation of cellular immunity and reduction of influenza virus titer in aged mice fed Lactobacillus casei strain Shirota. Clin Diagn Lab Immunol. 2002; 9(1):105-8. PMC: 119906. DOI: 10.1128/cdli.9.1.105-108.2002. View

19.

Al Kassaa I, Hamze M, Hober D, Chihib N, Drider D . Identification of vaginal lactobacilli with potential probiotic properties isolated from women in North Lebanon. Microb Ecol. 2014; 67(3):722-34. DOI: 10.1007/s00248-014-0384-7. View

20.

Quan F, Huang C, Compans R, Kang S . Virus-like particle vaccine induces protective immunity against homologous and heterologous strains of influenza virus. J Virol. 2007; 81(7):3514-24. PMC: 1866067. DOI: 10.1128/JVI.02052-06. View