Lactobacillus Plantarum DK119 As a Probiotic Confers Protection Against Influenza Virus by Modulating Innate Immunity

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Oct 15

PMID 24124485

Citations 101

Authors

Min-Kyung Park

Vu Ngo

Young-Man Kwon

Young-Tae Lee

Sieun Yoo

Young-Hee Cho

Sung-Moon Hong

Hye Suk Hwang

Eun-Ju Ko

Yu-Jin Jung

Dae-Won Moon

Eun-Ji Jeong

Min-Chul Kim

Yu-Na Lee

Ji-Hun Jang

Joon-Suk Oh

Cheol-Hyun Kim

Sang-Moo Kang

Affiliations

Soon will be listed here.

Abstract

Lactobacillus plantarum DK119 (DK119) isolated from the fermented Korean cabbage food was used as a probiotic to determine its antiviral effects on influenza virus. DK119 intranasal or oral administration conferred 100% protection against subsequent lethal infection with influenza A viruses, prevented significant weight loss, and lowered lung viral loads in a mouse model. The antiviral protective efficacy was observed in a dose and route dependent manner of DK119 administration. Mice that were treated with DK119 showed high levels of cytokines IL-12 and IFN-γ in bronchoalveolar lavage fluids, and a low degree of inflammation upon infection with influenza virus. Depletion of alveolar macrophage cells in lungs and bronchoalveolar lavages completely abrogated the DK119-mediated protection. Modulating host innate immunity of dendritic and macrophage cells, and cytokine production pattern appeared to be possible mechanisms by which DK119 exhibited antiviral effects on influenza virus infection. These results indicate that DK119 can be developed as a beneficial antiviral probiotic microorganism.

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References

Wang S, Le T, Kurihara N, Chida J, Cisse Y, Yano M . Influenza virus-cytokine-protease cycle in the pathogenesis of vascular hyperpermeability in severe influenza. J Infect Dis. 2010; 202(7):991-1001. PMC: 7537608. DOI: 10.1086/656044. View

Hori T, Kiyoshima J, Shida K, Yasui H . Effect of intranasal administration of Lactobacillus casei Shirota on influenza virus infection of upper respiratory tract in mice. Clin Diagn Lab Immunol. 2001; 8(3):593-7. PMC: 96107. DOI: 10.1128/CDLI.8.3.593-597.2001. View

Kobayashi N, Saito T, Uematsu T, Kishi K, Toba M, Kohda N . Oral administration of heat-killed Lactobacillus pentosus strain b240 augments protection against influenza virus infection in mice. Int Immunopharmacol. 2010; 11(2):199-203. DOI: 10.1016/j.intimp.2010.11.019. View

Kim M, Joo H . Immunostimulatory effects of fucoidan on bone marrow-derived dendritic cells. Immunol Lett. 2007; 115(2):138-43. DOI: 10.1016/j.imlet.2007.10.016. View

Bosio C, Dow S . Francisella tularensis induces aberrant activation of pulmonary dendritic cells. J Immunol. 2005; 175(10):6792-801. DOI: 10.4049/jimmunol.175.10.6792. View

Joo H . Altered maturation of dendritic cells by taxol, an anticancer drug. J Vet Sci. 2003; 4(3):229-34. View

Tumpey T, Garcia-Sastre A, Taubenberger J, Palese P, Swayne D, Pantin-Jackwood M . Pathogenicity of influenza viruses with genes from the 1918 pandemic virus: functional roles of alveolar macrophages and neutrophils in limiting virus replication and mortality in mice. J Virol. 2005; 79(23):14933-44. PMC: 1287592. DOI: 10.1128/JVI.79.23.14933-14944.2005. View

Van Reeth K . Cytokines in the pathogenesis of influenza. Vet Microbiol. 2000; 74(1-2):109-16. DOI: 10.1016/s0378-1135(00)00171-1. View

Hanniffy S, Wiedermann U, Repa A, Mercenier A, Daniel C, Fioramonti J . Potential and opportunities for use of recombinant lactic acid bacteria in human health. Adv Appl Microbiol. 2004; 56:1-64. DOI: 10.1016/S0065-2164(04)56001-X. View

10.

Nomura M, Kobayashi M, Narita T, Kimoto-Nira H, Okamoto T . Phenotypic and molecular characterization of Lactococcus lactis from milk and plants. J Appl Microbiol. 2006; 101(2):396-405. DOI: 10.1111/j.1365-2672.2006.02949.x. View

11.

Gorbach S . Lactic acid bacteria and human health. Ann Med. 1990; 22(1):37-41. DOI: 10.3109/07853899009147239. View

12.

Ishida Y, Nakamura F, Kanzato H, Sawada D, Hirata H, Nishimura A . Clinical effects of Lactobacillus acidophilus strain L-92 on perennial allergic rhinitis: a double-blind, placebo-controlled study. J Dairy Sci. 2005; 88(2):527-33. DOI: 10.3168/jds.S0022-0302(05)72714-4. View

13.

McGill J, van Rooijen N, Legge K . Protective influenza-specific CD8 T cell responses require interactions with dendritic cells in the lungs. J Exp Med. 2008; 205(7):1635-46. PMC: 2442641. DOI: 10.1084/jem.20080314. View

14.

Corr S, Gahan C, Hill C . Impact of selected Lactobacillus and Bifidobacterium species on Listeria monocytogenes infection and the mucosal immune response. FEMS Immunol Med Microbiol. 2007; 50(3):380-8. DOI: 10.1111/j.1574-695X.2007.00264.x. View

15.

Maeda N, Nakamura R, Hirose Y, Murosaki S, Yamamoto Y, Kase T . Oral administration of heat-killed Lactobacillus plantarum L-137 enhances protection against influenza virus infection by stimulation of type I interferon production in mice. Int Immunopharmacol. 2009; 9(9):1122-5. DOI: 10.1016/j.intimp.2009.04.015. View

16.

Hancock K, Veguilla V, Lu X, Zhong W, Butler E, Sun H . Cross-reactive antibody responses to the 2009 pandemic H1N1 influenza virus. N Engl J Med. 2009; 361(20):1945-52. DOI: 10.1056/NEJMoa0906453. View

17.

Drouault S, Corthier G . [Health effects of lactic acid bacteria ingested in fermented milk]. Vet Res. 2001; 32(2):101-17. DOI: 10.1051/vetres:2001115. View

18.

Dunne C, OMahony L, Murphy L, Thornton G, Morrissey D, OHalloran S . In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. Am J Clin Nutr. 2001; 73(2 Suppl):386S-392S. DOI: 10.1093/ajcn/73.2.386s. View

19.

Murawski M, McGinnes L, Finberg R, Kurt-Jones E, Massare M, Smith G . Newcastle disease virus-like particles containing respiratory syncytial virus G protein induced protection in BALB/c mice, with no evidence of immunopathology. J Virol. 2009; 84(2):1110-23. PMC: 2798376. DOI: 10.1128/JVI.01709-09. View

20.

Hall J, Woolard M, Gunn B, Craven R, Taft-Benz S, Frelinger J . Infected-host-cell repertoire and cellular response in the lung following inhalation of Francisella tularensis Schu S4, LVS, or U112. Infect Immun. 2008; 76(12):5843-52. PMC: 2583552. DOI: 10.1128/IAI.01176-08. View