High-Resolution Single Particle Zeta Potential Characterisation of Biological Nanoparticles Using Tunable Resistive Pulse Sensing

Overview

Journal Sci Rep

Specialty Science

Date 2017 Dec 14

PMID 29234015

Citations 43

Authors

Robert Vogel

Anoop K Pal

Siddharth Jambhrunkar

Pragnesh Patel

Sachin S Thakur

Eduardo Reategui

Harendra S Parekh

Paula Saa

Adonis Stassinopoulos

Murray F Broom

Affiliations

Soon will be listed here.

Abstract

Physicochemical properties of nanoparticles, such as size, shape, surface charge, density, and porosity play a central role in biological interactions and hence accurate determination of these characteristics is of utmost importance. Here we propose tunable resistive pulse sensing for simultaneous size and surface charge measurements on a particle-by-particle basis, enabling the analysis of a wide spectrum of nanoparticles and their mixtures. Existing methodologies for measuring zeta potential of nanoparticles using resistive pulse sensing are significantly improved by including convection into the theoretical model. The efficacy of this methodology is demonstrated for a range of biological case studies, including measurements of mixed anionic, cationic liposomes, extracellular vesicles in plasma, and in situ time study of DNA immobilisation on the surface of magnetic nanoparticles. The high-resolution single particle size and zeta potential characterisation will provide a better understanding of nano-bio interactions, positively impacting nanomedicine development and their regulatory approval.

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References

Kozak D, Anderson W, Vogel R, Chen S, Antaw F, Trau M . Simultaneous size and ζ-potential measurements of individual nanoparticles in dispersion using size-tunable pore sensors. ACS Nano. 2012; 6(8):6990-7. DOI: 10.1021/nn3020322. View

Whiteaker J, Zhao L, Zhang H, Feng L, Piening B, Anderson L . Antibody-based enrichment of peptides on magnetic beads for mass-spectrometry-based quantification of serum biomarkers. Anal Biochem. 2007; 362(1):44-54. PMC: 1852426. DOI: 10.1016/j.ab.2006.12.023. View

Blundell E, Vogel R, Platt M . Particle-by-Particle Charge Analysis of DNA-Modified Nanoparticles Using Tunable Resistive Pulse Sensing. Langmuir. 2016; 32(4):1082-90. DOI: 10.1021/acs.langmuir.5b03024. View

Ciaravino V, McCullough T, Cimino G, Sullivan T . Preclinical safety profile of plasma prepared using the INTERCEPT Blood System. Vox Sang. 2003; 85(3):171-82. DOI: 10.1046/j.1423-0410.2003.00351.x. View

Raposo G, Stoorvogel W . Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol. 2013; 200(4):373-83. PMC: 3575529. DOI: 10.1083/jcb.201211138. View

Sikora A, Shard A, Minelli C . Size and ζ-Potential Measurement of Silica Nanoparticles in Serum Using Tunable Resistive Pulse Sensing. Langmuir. 2016; 32(9):2216-24. DOI: 10.1021/acs.langmuir.5b04160. View

Pamme N, Wilhelm C . Continuous sorting of magnetic cells via on-chip free-flow magnetophoresis. Lab Chip. 2006; 6(8):974-80. DOI: 10.1039/b604542a. View

Willmott G, Vogel R, Yu S, Groenewegen L, Roberts G, Kozak D . Use of tunable nanopore blockade rates to investigate colloidal dispersions. J Phys Condens Matter. 2011; 22(45):454116. DOI: 10.1088/0953-8984/22/45/454116. View

Cevc G . How membrane chain melting properties are regulated by the polar surface of the lipid bilayer. Biochemistry. 1987; 26(20):6305-10. DOI: 10.1021/bi00394a002. View

10.

Arjmandi N, Van Roy W, Lagae L, Borghs G . Measuring the electric charge and zeta potential of nanometer-sized objects using pyramidal-shaped nanopores. Anal Chem. 2012; 84(20):8490-6. DOI: 10.1021/ac300705z. View

11.

DMello S, Cruz C, Chen M, Kapoor M, Lee S, Tyner K . The evolving landscape of drug products containing nanomaterials in the United States. Nat Nanotechnol. 2017; 12(6):523-529. DOI: 10.1038/nnano.2017.67. View

12.

Gresham D, Dunham M, Botstein D . Comparing whole genomes using DNA microarrays. Nat Rev Genet. 2008; 9(4):291-302. PMC: 7097741. DOI: 10.1038/nrg2335. View

13.

Kim H, Achermann M, Balet L, Hollingsworth J, Klimov V . Synthesis and characterization of Co/CdSe core/shell nanocomposites: bifunctional magnetic-optical nanocrystals. J Am Chem Soc. 2005; 127(2):544-6. DOI: 10.1021/ja047107x. View

14.

Vogel R, Willmott G, Kozak D, Roberts G, Anderson W, Groenewegen L . Quantitative sizing of nano/microparticles with a tunable elastomeric pore sensor. Anal Chem. 2011; 83(9):3499-506. DOI: 10.1021/ac200195n. View

15.

Thery C, Zitvogel L, Amigorena S . Exosomes: composition, biogenesis and function. Nat Rev Immunol. 2002; 2(8):569-79. DOI: 10.1038/nri855. View

16.

Ito T, Sun L, Crooks R . Simultaneous determination of the size and surface charge of individual nanoparticles using a carbon nanotube-based Coulter counter. Anal Chem. 2003; 75(10):2399-406. DOI: 10.1021/ac034072v. View

17.

Hall J, Dobrovolskaia M, Patri A, McNeil S . Characterization of nanoparticles for therapeutics. Nanomedicine (Lond). 2007; 2(6):789-803. DOI: 10.2217/17435889.2.6.789. View

18.

McCallister C, Kdeiss B, Nikolaidis N . Biochemical characterization of the interaction between HspA1A and phospholipids. Cell Stress Chaperones. 2015; 21(1):41-53. PMC: 4679732. DOI: 10.1007/s12192-015-0636-6. View

19.

Seddon J, Cevc G, Marsh D . Calorimetric studies of the gel-fluid (L beta-L alpha) and lamellar-inverted hexagonal (L alpha-HII) phase transitions in dialkyl- and diacylphosphatidylethanolamines. Biochemistry. 1983; 22(5):1280-9. DOI: 10.1021/bi00274a045. View

20.

Cocucci E, Racchetti G, Meldolesi J . Shedding microvesicles: artefacts no more. Trends Cell Biol. 2009; 19(2):43-51. DOI: 10.1016/j.tcb.2008.11.003. View