Skeletal Muscle Regenerative Potential of Human MuStem Cells Following Transplantation into Injured Mice Muscle

Overview

Journal Mol Ther

Publisher Cell Press

Specialties Molecular Biology
Pharmacology

Date 2017 Dec 10

PMID 29221805

Citations 19

Authors

Judith Lorant

Charlotte Saury

Cindy Schleder

Florence Robriquet

Blandine Lieubeau

Elisa Negroni

Isabelle Leroux

Lucie Chabrand

Sabrina Viau

Candice Babarit

Mireille Ledevin

Laurence Dubreil

Antoine Hamel

Armelle Magot

Chantal Thorin

Laetitia Guevel

Bruno Delorme

Yann Pereon

Gillian Butler-Browne

Vincent Mouly

Karl Rouger

Affiliations

Soon will be listed here.

Abstract

After intra-arterial delivery in the dystrophic dog, allogeneic muscle-derived stem cells, termed MuStem cells, contribute to long-term stabilization of the clinical status and preservation of the muscle regenerative process. However, it remains unknown whether the human counterpart could be identified, considering recent demonstrations of divergent features between species for several somatic stem cells. Here, we report that MuStem cells reside in human skeletal muscle and display a long-term ability to proliferate, allowing generation of a clinically relevant amount of cells. Cultured human MuStem (hMuStem) cells do not express hematopoietic, endothelial, or myo-endothelial cell markers and reproducibly correspond to a population of early myogenic-committed progenitors with a perivascular/mesenchymal phenotypic signature, revealing a blood vessel wall origin. Importantly, they exhibit both myogenesis in vitro and skeletal muscle regeneration after intramuscular delivery into immunodeficient host mice. Together, our findings provide new insights supporting the notion that hMuStem cells could represent an interesting therapeutic candidate for dystrophic patients.

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