Pre-microRNA Gene Polymorphisms and Risk of Cervical Squamous Cell Carcinoma

Overview

Journal J Clin Diagn Res

Specialty General Medicine

Date 2017 Dec 7

PMID 29207732

Citations 10

Authors

Shruti Srivastava

Shalini Singh

Naseem Fatima

Balraj Mittal

Anand Narain Srivastava

Affiliations

Soon will be listed here.

Abstract

Introduction: MicroRNAs (miRNAs) are short (~22 nucleotides) regulatory RNAs that can modulate gene expression and are aberrantly expressed in many diseases, including cancer. It has been suggested that, the presence of single nucleotide polymorphisms in precursor miRNAs (pre-miRNAs) can alter miRNA processing, expression and binding to target mRNA and represents another type of genetic variability, that can contribute to the susceptibility of human cancers.

Aim: The present study investigated the genetic variants in pre-miRNAs (hsa-miRNA-196a2 rs11614913 C/T, hsa-miRNA-499 rs3746444 T/C and hsa-miRNA-146a rs2910164 G/C) for their role in cervical cancer susceptibility.

Materials And Methods: The study comprised 164 controls and 184 patients of cervical cancer. The genotypic frequency of miRNA polymorphisms were determined by using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Logistic regression was used for statistical analysis using SPSS Software version 15.0.

Results: Hsa-miRNA-499 rs3746444 T/C polymorphism showed a statistically significant association with considerable risk for cervical cancer at genotypes (CC, p=0.001, OR=4.801) and variant allele (p<0.001, OR=2.307). MiRNA 146a and miRNA 196a2 polymorphisms showed no association with cervical cancer. However, interaction of miRNA polymorphisms with smoking habit showed higher risk of cervical cancer with miRNA 196a2 polymorphism in patients with smoking but no significant modification in the risk of cervical cancer was seen for other polymorphisms.

Conclusion: The results of the present study demonstrate that, miRNA 499 T/C polymorphism is significantly associated with genetic susceptibility to cervical cancer and may have a role in its pathogenesis.

Citing Articles

Rs3746444 T>C locus in miR-499 increases the susceptibility to hepatocellular carcinoma: A meta-analysis 14812 subjects.

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Effect of rs11614913 Polymorphism on Cancer Susceptibility: Evidence From an Updated Meta-Analysis.

Aziz M, Akter T, Islam M Technol Cancer Res Treat. 2022; 21:15330338221109798.

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Association between miR-146a rs2910164, miR-196a2 rs11614913, and miR-499 rs3746444 polymorphisms and the risk of esophageal carcinoma: A case-control study.

Liu C, Gao W, Shi Y, Lv L, Tang W Cancer Med. 2022; 11(21):3949-3959.

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Association of three micro-RNA gene polymorphisms with the risk of cervical cancer: a meta-analysis and systematic review.

Xu J, Geng J, Zhang Q, Fan Y, Qi Z, Xia T World J Surg Oncol. 2021; 19(1):346.

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The Association between Five Genetic Variants in MicroRNAs (rs2910164, rs11614913, rs3746444, rs11134527, and rs531564) and Cervical Cancer Risk: A Meta-Analysis.

Liu J, Dong P, Zhou L, Wang S Biomed Res Int. 2021; 2021:9180874.

PMID: 33816633 PMC: 7987420. DOI: 10.1155/2021/9180874.

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