The Colorectal MicroRNAome

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2006 Mar 1

PMID 16505370

Citations 439

Authors

Jordan M Cummins

Yiping He

Rebecca J Leary

Ray Pagliarini

Luis A Diaz Jr

Tobias Sjoblom

Omer Barad

Zvi Bentwich

Anna E Szafranska

Emmanuel Labourier

Christopher K Raymond

Brian S Roberts

Hartmut Juhl

Kenneth W Kinzler

Bert Vogelstein

Victor E Velculescu

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) are a class of small noncoding RNAs that have important regulatory roles in multicellular organisms. The public miRNA database contains 321 human miRNA sequences, 234 of which have been experimentally verified. To explore the possibility that additional miRNAs are present in the human genome, we have developed an experimental approach called miRNA serial analysis of gene expression (miRAGE) and used it to perform the largest experimental analysis of human miRNAs to date. Sequence analysis of 273,966 small RNA tags from human colorectal cells allowed us to identify 200 known mature miRNAs, 133 novel miRNA candidates, and 112 previously uncharacterized miRNA* forms. To aid in the evaluation of candidate miRNAs, we disrupted the Dicer locus in three human colorectal cancer cell lines and examined known and novel miRNAs in these cells. These studies suggest that the human genome contains many more miRNAs than currently identified and provide an approach for the large-scale experimental cloning of novel human miRNAs in human tissues.

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