Improved Detection of Cytokines Produced by Invariant NKT Cells

Overview

Journal Sci Rep

Specialty Science

Date 2017 Dec 2

PMID 29192280

Citations 16

Authors

Duygu Sag

Muge Ozkan

Mitchell Kronenberg

Gerhard Wingender

Affiliations

Soon will be listed here.

Abstract

Invariant Natural killer T (iNKT) cells rapidly produce copious amounts of multiple cytokines after in vivo activation, allowing for the direct detection of a number of cytokines directly ex vivo. However, for some cytokines this approach is suboptimal. Here, we report technical variations that allow the improved detection of IL-4, IL-10, IL-13 and IL-17A ex vivo. Furthermore, we describe an alternative approach for stimulation of iNKT cells in vitro that allows a significantly improved detection of cytokines produced by iNKT cells. Together, these protocols allow the detection of iNKT cell cytokines ex vivo and in vitro with increased sensitivity.

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