Gold Nanocage-based Lateral Flow Immunoassay for Immunoglobulin G

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2017 Dec 1

PMID 29187761

Citations 12

Authors

Yunhui Yang

Mehmet Ozsoz

Guodong Liu

Affiliations

Soon will be listed here.

Abstract

The authors describe a gold nanocage-based lateral flow strip biosensor (LFSB) for low-cost and sensitive detection of IgG. This protein was used as a model analyte to demonstrate the proof-of-concept. The method combines the unique optical properties of gold nanocages (GNCs) with highly efficient chromatographic separation. A sandwich-type of immunoreactions occurs on the GNC-based LFSB which has the attractive features of avoiding multiple incubation, separation, and washing steps. The captured GNCs on the purple test zone and control zone of the biosensor are producing characteristic purple bands, and this enables IgG even to be visually detected. Quantitatation was accomplished by reading the intensities of the bands with a portable strip reader. The LFSB fabrication and assay parameters were optimized. The biosensor displays a linear response in the 0.5 to 50 ng·mL IgG concentration range, and it has a 15 min assay time. The detection limit is 0.1 ng·mL of IgG, which is 2.5 times lower than that when using a gold nanoparticle-based LFSB. In our perception, this assay has a wide potential for the detection of other proteins and species for which respective antibodies are available.

Citing Articles

Unleashing the power of colloidal gold immunochromatographic assays for plant virus diagnostics.

Ghorbani A, Astaraki S, Rostami M, Pakdel A MethodsX. 2023; 12:102498.

PMID: 38089155 PMC: 10714363. DOI: 10.1016/j.mex.2023.102498.

High-Sensitive Detection and Quantitative Analysis of Thyroid-Stimulating Hormone Using Gold-Nanoshell-Based Lateral Flow Immunoassay Device.

Bikkarolla S, McNamee S, Vance P, McLaughlin J Biosensors (Basel). 2022; 12(3).

PMID: 35323452 PMC: 8946628. DOI: 10.3390/bios12030182.

Construction and Signal Feature Processing of Gold Nanobiosensors Based on the Internet of Things.

Chen L J Healthc Eng. 2022; 2022:1432266.

PMID: 35047147 PMC: 8763554. DOI: 10.1155/2022/1432266.

Tailoring noble metal nanoparticle designs to enable sensitive lateral flow immunoassay.

Chen X, Ding L, Huang X, Xiong Y Theranostics. 2022; 12(2):574-602.

PMID: 34976202 PMC: 8692915. DOI: 10.7150/thno.67184.

A rapid assay provides on-site quantification of tetrahydrocannabinol in oral fluid.

Yu H, Lee H, Cheong J, Woo S, Oh J, Oh H Sci Transl Med. 2021; 13(616):eabe2352.

PMID: 34669441 PMC: 9126021. DOI: 10.1126/scitranslmed.abe2352.

References

Skrabalak S, Au L, Li X, Xia Y . Facile synthesis of Ag nanocubes and Au nanocages. Nat Protoc. 2007; 2(9):2182-90. DOI: 10.1038/nprot.2007.326. View

Zhang M, Wang M, Chen Z, Fang J, Fang M, Liu J . Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine. Anal Bioanal Chem. 2009; 395(8):2591-9. DOI: 10.1007/s00216-009-3181-2. View

Skrabalak S, Chen J, Sun Y, Lu X, Au L, Cobley C . Gold nanocages: synthesis, properties, and applications. Acc Chem Res. 2008; 41(12):1587-95. PMC: 2645935. DOI: 10.1021/ar800018v. View

Zhao Y, Zhang G, Liu Q, Teng M, Yang J, Wang J . Development of a lateral flow colloidal gold immunoassay strip for the rapid detection of enrofloxacin residues. J Agric Food Chem. 2008; 56(24):12138-42. DOI: 10.1021/jf802648z. View

Wang S, Chen Z, Choo J, Chen L . Naked-eye sensitive ELISA-like assay based on gold-enhanced peroxidase-like immunogold activity. Anal Bioanal Chem. 2015; 408(4):1015-22. DOI: 10.1007/s00216-015-9219-8. View

He Y, Zeng K, Gurung A, Baloda M, Xu H, Zhang X . Visual detection of single-nucleotide polymorphism with hairpin oligonucleotide-functionalized gold nanoparticles. Anal Chem. 2010; 82(17):7169-77. DOI: 10.1021/ac101275s. View

Gussenhoven G, van der Hoorn M, Goris M, Terpstra W, Hartskeerl R, Mol B . LEPTO dipstick, a dipstick assay for detection of Leptospira-specific immunoglobulin M antibodies in human sera. J Clin Microbiol. 1997; 35(1):92-7. PMC: 229517. DOI: 10.1128/jcm.35.1.92-97.1997. View

Mao X, Ma Y, Zhang A, Zhang L, Zeng L, Liu G . Disposable nucleic acid biosensors based on gold nanoparticle probes and lateral flow strip. Anal Chem. 2009; 81(4):1660-8. DOI: 10.1021/ac8024653. View

Lopez A, Lovato F, Oh S, Lai Y, Filbrun S, Driskell E . SERS immunoassay based on the capture and concentration of antigen-assembled gold nanoparticles. Talanta. 2015; 146:388-93. DOI: 10.1016/j.talanta.2015.08.065. View

10.

PLOTZ C, SINGER J . The latex fixation test. I. Application to the serologic diagnosis of rheumatoid arthritis. Am J Med. 1956; 21(6):888-92. View

11.

Peng Z, Chen Z, Jiang J, Zhang X, Shen G, Yu R . A novel immunoassay based on the dissociation of immunocomplex and fluorescence quenching by gold nanoparticles. Anal Chim Acta. 2007; 583(1):40-4. DOI: 10.1016/j.aca.2006.10.006. View

12.

Xu H, Mao X, Zeng Q, Wang S, Kawde A, Liu G . Aptamer-functionalized gold nanoparticles as probes in a dry-reagent strip biosensor for protein analysis. Anal Chem. 2008; 81(2):669-75. DOI: 10.1021/ac8020592. View

13.

Zou Z, Du D, Wang J, Smith J, Timchalk C, Li Y . Quantum dot-based immunochromatographic fluorescent biosensor for biomonitoring trichloropyridinol, a biomarker of exposure to chlorpyrifos. Anal Chem. 2010; 82(12):5125-33. DOI: 10.1021/ac100260m. View

14.

Elghanian R, Storhoff J, Mucic R, LETSINGER R, Mirkin C . Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles. Science. 1997; 277(5329):1078-81. DOI: 10.1126/science.277.5329.1078. View

15.

Wang L, Wang S, Zhang J, Liu J, Zhang Y . Enzyme-linked immunosorbent assay and colloidal gold immunoassay for sulphamethazine residues in edible animal foods: investigation of the effects of the analytical conditions and the sample matrix on assay performance. Anal Bioanal Chem. 2008; 390(6):1619-27. DOI: 10.1007/s00216-008-1836-z. View

16.

Lonnberg M, Carlsson J . Quantitative detection in the attomole range for immunochromatographic tests by means of a flatbed scanner. Anal Biochem. 2001; 293(2):224-31. DOI: 10.1006/abio.2001.5130. View

17.

Birnbaum S, Uden C, Magnusson C, Nilsson S . Latex-based thin-layer immunoaffinity chromatography for quantitation of protein analytes. Anal Biochem. 1992; 206(1):168-71. DOI: 10.1016/s0003-2697(05)80028-4. View

18.

Zheng M, Richard J, Binder J . A review of rapid methods for the analysis of mycotoxins. Mycopathologia. 2006; 161(5):261-73. DOI: 10.1007/s11046-006-0215-6. View

19.

Chen Y, Wang Z, Wang Z, Tang S, Zhu Y, Xiao X . Rapid enzyme-linked immunosorbent assay and colloidal gold immunoassay for kanamycin and tobramycin in Swine tissues. J Agric Food Chem. 2008; 56(9):2944-52. DOI: 10.1021/jf703602b. View

20.

Zhang D, Sun Y, Wu Q, Ma P, Zhang H, Wang Y . Enhancing sensitivity of surface plasmon resonance biosensor by Ag nanocubes/chitosan composite for the detection of mouse IgG. Talanta. 2015; 146:364-8. DOI: 10.1016/j.talanta.2015.08.050. View