ALKBH10B Is an RNA -Methyladenosine Demethylase Affecting Arabidopsis Floral Transition

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 2017 Nov 29

PMID 29180595

Citations 159

Authors

Hong-Chao Duan

Lian-Huan Wei

Chi Zhang

Ye Wang

Lin Chen

Zhike Lu

Peng R Chen

Chuan He

Guifang Jia

Affiliations

Soon will be listed here.

Abstract

-methyladenosine (mA) is the most abundant, internal, posttranscriptional modification in mRNA among all higher eukaryotes. In mammals, this modification is reversible and plays broad roles in the regulation of mRNA metabolism and processing. Despite its importance, previous studies on the role and mechanism of mA methylation in have been limited. Here, we report that ALKBH10B is a demethylase that oxidatively reverses mA methylation in mRNA in vitro and in vivo. Depletion of ALKBH10B in the mutant delays flowering and represses vegetative growth. Complementation with wild-type , but not a catalytically inactive mutant (), rescues these effects in mutant plants, suggesting the observed phenotypes are controlled by the catalytic action of We show that -mediated mRNA demethylation affects the stability of target transcripts, thereby influencing floral transition. We identified 1190 mA hypermethylated transcripts in the mutant involved in plant development. The discovery and characterization of the archetypical RNA demethylase in Arabidopsis sheds light on the occurrence and functional role(s) of reversible mRNA methylation in plants and defines the role of mA RNA modification in Arabidopsis floral transition.

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