Genome-guided Identification of Novel Head-to-tail Cyclized Antimicrobial Peptides, Exemplified by the Discovery of Pumilarin

Overview

Journal Microb Genom

Specialties Genetics
Microbiology

Date 2017 Nov 28

PMID 29177092

Citations 25

Authors

Auke J van Heel

Manuel Montalban-Lopez

Quentin Oliveau

Oscar P Kuipers

Affiliations

Soon will be listed here.

Abstract

The need for novel antibiotics in an era where antimicrobial resistance is on the rise, and the number of new approved antimicrobial drugs reaching the market is declining, is evident. The underused potential of post-translationally modified peptides for clinical use makes this class of peptides interesting candidates. In this study, we made use of the vast amounts of available genomic data and screened all publicly available prokaryotic genomes (~3000) to identify 394 novel head-to-tail cyclized antimicrobial peptides. To verify these results, we isolated and characterized a novel antimicrobial peptide from that we named pumilarin. Pumilarin was demonstrated to have a circular structure and showed antimicrobial activity against several indicator strains, including pathogens.

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