MafB is a Critical Regulator of Complement Component C1q

Overview

Journal Nat Commun

Specialty Biology

Date 2017 Nov 24

PMID 29167450

Citations 39

Authors

Mai Thi Nhu Tran

Michito Hamada

Hyojung Jeon

Risako Shiraishi

Keigo Asano

Motochika Hattori

Megumi Nakamura

Yuki Imamura

Yuki Tsunakawa

Risa Fujii

Toshiaki Usui

Kaushalya Kulathunga

Christina-Sylvia Andrea

Ryusuke Koshida

Risa Kamei

Yurina Matsunaga

Makoto Kobayashi

Hisashi Oishi

Takashi Kudo

Satoru Takahashi

Affiliations

Soon will be listed here.

Abstract

The transcription factor MafB is expressed by monocytes and macrophages. Efferocytosis (apoptotic cell uptake) by macrophages is important for inhibiting the development of autoimmune diseases, and is greatly reduced in Mafb-deficient macrophages. Here, we show the expression of the first protein in the classical complement pathway C1q is important for mediating efferocytosis and is reduced in Mafb-deficient macrophages. The efferocytosis defect in Mafb-deficient macrophages can be rescued by adding serum from wild-type mice, but not by adding serum from C1q-deficient mice. By hemolysis assay we also show that activation of the classical complement pathway is decreased in Mafb-deficient mice. In addition, MafB overexpression induces C1q-dependent gene expression and signals that induce C1q genes are less effective in the absence of MafB. We also show that Mafb-deficiency can increase glomerular autoimmunity, including anti-nuclear antibody deposition. These results show that MafB is an important regulator of C1q.

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