Self-assembly of Nucleopeptides to Interact with DNAs

Overview

Journal Interface Focus

Specialty Biology

Date 2017 Nov 18

PMID 29147550

Citations 11

Authors

Xuewen Du

Jie Zhou

Xinming Li

Bing Xu

Affiliations

Soon will be listed here.

Abstract

As a novel class of biomaterials, nucleopeptides, via the conjugation of nucleobases and peptides, usually self-assemble to form nanofibres driven mainly by hydrogen bonds. Containing nucleobase(s), nucleopeptides have a unique property-interacting with nucleic acids. Here we report the design and characterization of nucleopeptides that self-assemble in water and are able to interact with single-stranded DNAs (ssDNAs). Containing nucleobases on their side chains, these nucleopeptides bind with the ssDNAs, and the ssDNAs reciprocally affect the self-assembly of nucleopeptides. In addition, the interactions between nucleopeptides and ssDNAs also decrease their proteolytic resistance against proteinase K, which further demonstrates the binding with ssDNAs. The nucleopeptides also interact with plasmid DNA and deliver hairpin DNA into cells. This work illustrates a new and rational approach to create soft biomaterials by the integration of nucleobases and peptides to bind with DNA, which may lead to the development of nucleopeptides for controlling DNA in cells.

Citing Articles

Solid Phase Synthesis and TAR RNA-Binding Activity of Nucleopeptides Containing Nucleobases Linked to the Side Chains via 1,4-Linked-1,2,3-triazole.

Mucha P, Pieszko M, Bylinska I, Wiczk W, Ruczynski J, Prochera K Biomedicines. 2024; 12(3).

PMID: 38540183 PMC: 10968536. DOI: 10.3390/biomedicines12030570.

Dancing with Nucleobases: Unveiling the Self-Assembly Properties of DNA and RNA Base-Containing Molecules for Gel Formation.

Scognamiglio P, Vicidomini C, Roviello G Gels. 2024; 10(1).

PMID: 38247739 PMC: 10815473. DOI: 10.3390/gels10010016.

Tryptophan-PNA gc Conjugates Self-Assemble to Form Fibers.

Mosseri A, Sancho-Albero M, Mercurio F, Leone M, De Cola L, Romanelli A Bioconjug Chem. 2023; 34(8):1429-1438.

PMID: 37486977 PMC: 10436247. DOI: 10.1021/acs.bioconjchem.3c00200.

Diversity of Bioinspired Hydrogels: From Structure to Applications.

Lupu A, Gradinaru L, Gradinaru V, Bercea M Gels. 2023; 9(5).

PMID: 37232968 PMC: 10217308. DOI: 10.3390/gels9050376.

Intramitochondrial co-assembly between ATP and nucleopeptides induces cancer cell apoptosis.

Choi H, Park G, Shin E, Shin S, Jana B, Jin S Chem Sci. 2022; 13(21):6197-6204.

PMID: 35733910 PMC: 9159100. DOI: 10.1039/d1sc05738c.

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