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Generation of Human IPSC Line from a Patient with Laterality Defects and Associated Congenital Heart Anomalies Carrying a DAND5 Missense Alteration

Overview
Journal Stem Cell Res
Publisher Elsevier
Specialty Cell Biology
Date 2017 Nov 15
PMID 29136563
Citations 7
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Abstract

A human iPSC line was generated from exfoliated renal epithelial (ERE) cells of a patient affected with Congenital Heart Disease (CHD) and Laterality Defects carrying tshe variant p.R152H in the DAND5 gene. The transgene-free iPSCs were generated with the human OSKM transcription factor using the Sendai-virus reprogramming system. The established iPSC line had the specific heterozygous alteration, a stable karyotype, expressed pluripotency markers and generated embryoid bodies that can differentiate towards the three germ layers in vitro. This iPSC line offers a useful resource to study the molecular mechanisms of cardiomyocyte proliferation, as well as for drug testing.

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