MiR-361-5p Inhibits Glycolytic Metabolism, Proliferation and Invasion of Breast Cancer by Targeting FGFR1 and MMP-1

Overview

Journal J Exp Clin Cancer Res

Publisher Biomed Central

Specialty Oncology

Date 2017 Nov 15

PMID 29132384

Citations 37

Authors

Fei Ma

Lei Zhang

Li Ma

Yiyun Zhang

Jianguo Zhang

Baoliang Guo

Affiliations

Soon will be listed here.

Abstract

Background: MicroRNAs function as key regulators in various human cancers, including breast cancer (BC). MiR-361-5p has been proved to be a tumor suppressor in colorectal cancer and gastric cancer in our previous study. In this study, we aim to find out the function of miR-361-5p in breast cancer progression and elaborate the mechanism that miR-361-5p acts its function in breast cancer.

Methods And Results: Here we reported that miR-361-5p was down-regulated in breast cancer tissue compared with normal breast tissue and the expression of miR-361-5p was positively associated with prognosis in BC patients. Functional studies showed that overexpression of miR-361-5p suppressed the proliferation, invasion and metastasis of breast cancer cells both in vivo and in vitro. Mechanistically, we found that miR-361-5p inhibited the proliferation of BC cells by suppressing glycolysis. FGFR1, a promoter of glycolysis-related enzyme, was identified as the target of miR-361-5p that promoted glycolysis and repressed oxidative phosphorylation. Furthermore, we demonstrated that miR-361-5p inhibited breast cancer cells invasion and metastasis by targeting MMP-1. An inverse expression pattern was also found between miR-361-5p and FGFR1 or MMP-1 in a cohort of 60 BC tissues.

Conclusion: Our results indicate that miR-361-5p inhibits breast cancer cells glycolysis and invasion by respectively repressing FGFR1 and MMP-1, suggesting that miR-361-5p and its targets may serve as therapeutic targets in breast cancer treatment.

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References

Hong X, Xu Y, Qiu X, Zhu Y, Feng X, Ding Z . MiR-448 promotes glycolytic metabolism of gastric cancer by downregulating KDM2B. Oncotarget. 2016; 7(16):22092-102. PMC: 5008346. DOI: 10.18632/oncotarget.8020. View

Fan J, Hitosugi T, Chung T, Xie J, Ge Q, Gu T . Tyrosine phosphorylation of lactate dehydrogenase A is important for NADH/NAD(+) redox homeostasis in cancer cells. Mol Cell Biol. 2011; 31(24):4938-50. PMC: 3233034. DOI: 10.1128/MCB.06120-11. View

Gross J, Lapiere C . Collagenolytic activity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci U S A. 1962; 48:1014-22. PMC: 220898. DOI: 10.1073/pnas.48.6.1014. View

Warburg O . On the origin of cancer cells. Science. 1956; 123(3191):309-14. DOI: 10.1126/science.123.3191.309. View

Perez-Garcia E, Meza-Sosa K, Lopez-Sevilla Y, Camacho-Concha N, Sanchez N, Perez-Martinez L . Merlin negative regulation by miR-146a promotes cell transformation. Biochem Biophys Res Commun. 2015; 468(4):594-600. DOI: 10.1016/j.bbrc.2015.10.156. View

Cairns R, Harris I, Mak T . Regulation of cancer cell metabolism. Nat Rev Cancer. 2011; 11(2):85-95. DOI: 10.1038/nrc2981. View

Diaz-Lopez A, Moreno-Bueno G, Cano A . Role of microRNA in epithelial to mesenchymal transition and metastasis and clinical perspectives. Cancer Manag Res. 2014; 6:205-16. PMC: 4008290. DOI: 10.2147/CMAR.S38156. View

Tiburcio M, Costa S, DE Fatima Duarte M, Schmitt F, Longatto Filho A . Characterization of PAR1 and FGFR1 expression in invasive breast carcinomas: Prognostic significance. Oncol Lett. 2012; 4(4):647-657. PMC: 3506650. DOI: 10.3892/ol.2012.806. View

Hong W, Dong E . The past, present and future of breast cancer research in China. Cancer Lett. 2014; 351(1):1-5. DOI: 10.1016/j.canlet.2014.04.007. View

10.

Courtnay R, Ngo D, Malik N, Ververis K, Tortorella S, Karagiannis T . Cancer metabolism and the Warburg effect: the role of HIF-1 and PI3K. Mol Biol Rep. 2015; 42(4):841-51. DOI: 10.1007/s11033-015-3858-x. View

11.

Rakoff-Nahoum S . Why cancer and inflammation?. Yale J Biol Med. 2007; 79(3-4):123-30. PMC: 1994795. View

12.

Han M, Liu M, Wang Y, Mo Z, Bi X, Liu Z . Re-expression of miR-21 contributes to migration and invasion by inducing epithelial-mesenchymal transition consistent with cancer stem cell characteristics in MCF-7 cells. Mol Cell Biochem. 2011; 363(1-2):427-36. DOI: 10.1007/s11010-011-1195-5. View

13.

Eck S, Hoopes P, Petrella B, Coon C, Brinckerhoff C . Matrix metalloproteinase-1 promotes breast cancer angiogenesis and osteolysis in a novel in vivo model. Breast Cancer Res Treat. 2008; 116(1):79-90. PMC: 3772530. DOI: 10.1007/s10549-008-0085-3. View

14.

Aguer C, Gambarotta D, Mailloux R, Moffat C, Dent R, McPherson R . Galactose enhances oxidative metabolism and reveals mitochondrial dysfunction in human primary muscle cells. PLoS One. 2011; 6(12):e28536. PMC: 3240634. DOI: 10.1371/journal.pone.0028536. View

15.

Lo A, Dawson C, Young L, Ko C, Hau P, Lo K . Activation of the FGFR1 signalling pathway by the Epstein-Barr virus-encoded LMP1 promotes aerobic glycolysis and transformation of human nasopharyngeal epithelial cells. J Pathol. 2015; 237(2):238-48. DOI: 10.1002/path.4575. View

16.

Justus C, Sanderlin E, Yang L . Molecular Connections between Cancer Cell Metabolism and the Tumor Microenvironment. Int J Mol Sci. 2015; 16(5):11055-86. PMC: 4463690. DOI: 10.3390/ijms160511055. View

17.

Kanitz A, Imig J, Dziunycz P, Primorac A, Galgano A, Hofbauer G . The expression levels of microRNA-361-5p and its target VEGFA are inversely correlated in human cutaneous squamous cell carcinoma. PLoS One. 2012; 7(11):e49568. PMC: 3498195. DOI: 10.1371/journal.pone.0049568. View

18.

Hou X, Sun X, Yu Y, Zhao H, Yang Z, Wang X . miR-361-5p suppresses lung cancer cell lines progression by targeting FOXM1. Neoplasma. 2017; 64(4):526-534. DOI: 10.4149/neo_2017_406. View

19.

Hitosugi T, Fan J, Chung T, Lythgoe K, Wang X, Xie J . Tyrosine phosphorylation of mitochondrial pyruvate dehydrogenase kinase 1 is important for cancer metabolism. Mol Cell. 2011; 44(6):864-77. PMC: 3246218. DOI: 10.1016/j.molcel.2011.10.015. View

20.

Ma F, Zhang J, Zhong L, Wang L, Liu Y, Wang Y . Upregulated microRNA-301a in breast cancer promotes tumor metastasis by targeting PTEN and activating Wnt/β-catenin signaling. Gene. 2013; 535(2):191-7. DOI: 10.1016/j.gene.2013.11.035. View