Methodological Standards for In vitro Models of Epilepsy and Epileptic Seizures. A TASK1-WG4 Report of the AES/ILAE Translational Task Force of the ILAE

Overview

Journal Epilepsia

Specialty Neurology

Date 2017 Nov 7

PMID 29105075

Citations 19

Authors

Joseph V Raimondo

Uwe Heinemann

Marco de Curtis

Howard P Goodkin

Chris G Dulla

Damir Janigro

Akio Ikeda

Chou-Ching K Lin

Premysl Jiruska

Aristea S Galanopoulou

Christophe Bernard

Affiliations

Soon will be listed here.

Abstract

In vitro preparations are a powerful tool to explore the mechanisms and processes underlying epileptogenesis and ictogenesis. In this review, we critically review the numerous in vitro methodologies utilized in epilepsy research. We provide support for the inclusion of detailed descriptions of techniques, including often ignored parameters with unpredictable yet significant effects on study reproducibility and outcomes. In addition, we explore how recent developments in brain slice preparation relate to their use as models of epileptic activity.

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References

Herzog A, Harden C, Liporace J, Pennell P, Schomer D, Sperling M . Frequency of catamenial seizure exacerbation in women with localization-related epilepsy. Ann Neurol. 2004; 56(3):431-4. DOI: 10.1002/ana.20214. View

Matos G, Andersen M, Valle A, Tufik S . The relationship between sleep and epilepsy: evidence from clinical trials and animal models. J Neurol Sci. 2010; 295(1-2):1-7. DOI: 10.1016/j.jns.2010.05.003. View

Morris G, Jiruska P, Jefferys J, Powell A . A New Approach of Modified Submerged Patch Clamp Recording Reveals Interneuronal Dynamics during Epileptiform Oscillations. Front Neurosci. 2016; 10:519. PMC: 5101843. DOI: 10.3389/fnins.2016.00519. View

De Simoni A, Yu L . Preparation of organotypic hippocampal slice cultures: interface method. Nat Protoc. 2007; 1(3):1439-45. DOI: 10.1038/nprot.2006.228. View

Singer W, Lux H . Extracellular potassium gradients and visual receptive fields in the cat striate cortex. Brain Res. 1975; 96(2):378-83. DOI: 10.1016/0006-8993(75)90751-9. View

Aghajanian G, Rasmussen K . Intracellular studies in the facial nucleus illustrating a simple new method for obtaining viable motoneurons in adult rat brain slices. Synapse. 1989; 3(4):331-8. DOI: 10.1002/syn.890030406. View

Zhao S, Ting J, Atallah H, Qiu L, Tan J, Gloss B . Cell type–specific channelrhodopsin-2 transgenic mice for optogenetic dissection of neural circuitry function. Nat Methods. 2011; 8(9):745-52. PMC: 3191888. DOI: 10.1038/nmeth.1668. View

Jackson J, Amilhon B, Goutagny R, Bott J, Manseau F, Kortleven C . Reversal of theta rhythm flow through intact hippocampal circuits. Nat Neurosci. 2014; 17(10):1362-70. DOI: 10.1038/nn.3803. View

Williamson A, Ferro M, Leleux P, Ismailova E, Kaszas A, Doublet T . Localized Neuron Stimulation with Organic Electrochemical Transistors on Delaminating Depth Probes. Adv Mater. 2015; 27(30):4405-4410. DOI: 10.1002/adma.201500218. View

10.

Kuenzi F, Fitzjohn S, Morton R, Collingridge G, Seabrook G . Reduced long-term potentiation in hippocampal slices prepared using sucrose-based artificial cerebrospinal fluid. J Neurosci Methods. 2000; 100(1-2):117-22. DOI: 10.1016/s0165-0270(00)00239-9. View

11.

Debanne D, Guerineau N, Gahwiler B, Thompson S . Physiology and pharmacology of unitary synaptic connections between pairs of cells in areas CA3 and CA1 of rat hippocampal slice cultures. J Neurophysiol. 1995; 73(3):1282-94. DOI: 10.1152/jn.1995.73.3.1282. View

12.

Mainen Z, Maletic-Savatic M, Shi S, Hayashi Y, Malinow R, Svoboda K . Two-photon imaging in living brain slices. Methods. 1999; 18(2):231-9, 181. DOI: 10.1006/meth.1999.0776. View

13.

PASTERNACK M, Smirnov S, Kaila K . Proton modulation of functionally distinct GABAA receptors in acutely isolated pyramidal neurons of rat hippocampus. Neuropharmacology. 1996; 35(9-10):1279-88. DOI: 10.1016/s0028-3908(96)00075-5. View

14.

Quigg M, Clayburn H, Straume M, Menaker M, Bertram 3rd E . Effects of circadian regulation and rest-activity state on spontaneous seizures in a rat model of limbic epilepsy. Epilepsia. 2000; 41(5):502-9. DOI: 10.1111/j.1528-1157.2000.tb00202.x. View

15.

Mody I, Lambert J, Heinemann U . Low extracellular magnesium induces epileptiform activity and spreading depression in rat hippocampal slices. J Neurophysiol. 1987; 57(3):869-88. DOI: 10.1152/jn.1987.57.3.869. View

16.

Pomper J, GRAULICH J, Kovacs R, Hoffmann U, Gabriel S, Heinemann U . High oxygen tension leads to acute cell death in organotypic hippocampal slice cultures. Brain Res Dev Brain Res. 2001; 126(1):109-16. DOI: 10.1016/s0165-3806(00)00132-2. View

17.

McKhann 2nd G, Wenzel H, Robbins C, Sosunov A, Schwartzkroin P . Mouse strain differences in kainic acid sensitivity, seizure behavior, mortality, and hippocampal pathology. Neuroscience. 2003; 122(2):551-61. DOI: 10.1016/s0306-4522(03)00562-1. View

18.

Karus C, Mondragao M, Ziemens D, Rose C . Astrocytes restrict discharge duration and neuronal sodium loads during recurrent network activity. Glia. 2015; 63(6):936-57. DOI: 10.1002/glia.22793. View

19.

Alfonsa H, Merricks E, Codadu N, Cunningham M, Deisseroth K, Racca C . The contribution of raised intraneuronal chloride to epileptic network activity. J Neurosci. 2015; 35(20):7715-26. PMC: 4438123. DOI: 10.1523/JNEUROSCI.4105-14.2015. View

20.

Boyden E, Zhang F, Bamberg E, Nagel G, Deisseroth K . Millisecond-timescale, genetically targeted optical control of neural activity. Nat Neurosci. 2005; 8(9):1263-8. DOI: 10.1038/nn1525. View