Mapping Platinum Adducts on Yeast Ribosomal RNA Using High-throughput Sequencing

Overview

Journal Chem Commun (Camb)

Publisher Royal Society of Chemistry

Specialty Chemistry

Date 2017 Nov 4

PMID 29099140

Citations 6

Authors

Kory Plakos

Victoria J DeRose

Affiliations

Soon will be listed here.

Abstract

Methods to map small-molecule binding sites on cellular RNAs are important for understanding interactions with both endogenous and exogenous compounds. Pt(ii) reagents are well-known DNA and RNA crosslinking agents, but sequence-specific and genome-wide identification of Pt targets following in-cell treatment is challenging. Here we describe application of high-throughput 'Pt-Seq' to identify Pt-rRNA adducts following treatment of S. cerevisiae with cisplatin.

Citing Articles

The unique Pt(II)-induced nucleolar stress response and its deviation from DNA damage response pathways.

Pigg H, Alley K, Griffin C, Moon C, Kraske S, DeRose V J Biol Chem. 2024; 300(11):107858.

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Inhibition of nucleolar transcription by oxaliplatin involves ATM/ATR kinase signaling.

Nechay M, Wang D, Kleiner R Cell Chem Biol. 2023; 30(8):906-919.e4.

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Human ribosomal G-quadruplexes regulate heme bioavailability.

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Nucleolar Stress Induction by Oxaliplatin and Derivatives.

Sutton E, McDevitt C, Prochnau J, Yglesias M, Mroz A, Yang M J Am Chem Soc. 2019; 141(46):18411-18415.

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Amino acid-linked platinum(II) compounds: non-canonical nucleoside preferences and influence on glycosidic bond stabilities.

Kimutai B, He C, Roberts A, Jones M, Bao X, Jiang J J Biol Inorg Chem. 2019; 24(7):985-997.

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