Glutaraldehyde Test for the Rapid Diagnosis of Pulmonary and Extra-pulmonary Tuberculosis in an Area with High Tuberculosis Incidence

Overview

Journal Mem Inst Oswaldo Cruz

Specialties Parasitology
Tropical Medicine

Date 2017 Nov 2

PMID 29091139

Citations 2

Authors

Ben Hadj Hassine Ahmed

Marzouk Manel

Dhaou Mohamed

Boukadida Jalel

Affiliations

Soon will be listed here.

Abstract

Background: Tuberculosis (TB) remains one of the leading causes of morbidity and mortality worldwide. The primary method for controlling TB is the rapid and accurate identification of infected individuals. Immune response exploitation represents one of the main methods used for early TB diagnosis; however, few studies have reported that whole blood originating from TB-infected patients gels faster in the presence of aldehyde than blood originating from healthy subjects, which is the focus of the current study.

Objectives: The study objectives are to determine the diagnostic value of a glutaraldehyde test (GT) in pulmonary tuberculosis (PTB) and extra-pulmonary tuberculosis (EPTB) and to assess its performance compared with light-emitting diode fluorescence microscopy (LED-FM).

Materials And Methods: This study included 272 specimens (176 suspected PTB specimens and 96 suspected EPTB specimens). Of the 272 patients, 98 patients had TB infection confirmed by culture (64 PTB cases and 34 EPTB cases), and 174 patients had no TB infection. The gold standard technique (culture) was used as reference to verify the GT's performance.

Results: The GT showed a high sensitivity (96.9%) and specificity (82.1%) for PTB with a good positive predictive value (PPV = 75.6%) and negative predictive value (NPV = 97.9%). For EPTB, the GT showed a sensitivity of 91.2% and a specificity of 77.4%, with PPV = 68.9% and NPV = 94.1%. LED-FM had lower sensitivities for PTB (65.6%) and EPTB (42.1%) and an excellent specificity of 100%, with PPV = 100% and NPV = 100%.

Conclusion: We concluded that GT is rapid, easy, simple and cost-effective and does not require qualified personnel with a specific background or sophisticated equipment like molecular biology or mycobacterium-specific genotyping techniques. These qualities make the GT attractive for use in low- and high-income countries in addition to other conventional methods, particularly culture, which continues to be the gold standard.

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References

Sarode R, Bhasin D, Marwaha N, Roy P, Singh K, Panigrahi D . Hyperaggregation of platelets in intestinal tuberculosis: role of platelets in chronic inflammation. Am J Hematol. 1995; 48(1):52-4. DOI: 10.1002/ajh.2830480111. View

Affolabi D, Torrea G, Odoun M, Senou N, Ali Ligali M, Anagonou S . Comparison of two LED fluorescence microscopy build-on modules for acid-fast smear microscopy. Int J Tuberc Lung Dis. 2010; 14(2):160-4. View

Buyukasik Y, Soylu B, Soylu A, Ozcebe O, Canbakan S, Haznedaroglu I . In vivo platelet and T-lymphocyte activities during pulmonary tuberculosis. Eur Respir J. 1999; 12(6):1375-9. DOI: 10.1183/09031936.98.12061375. View

Deggim-Messmer V, Bloemberg G, Ritter C, Voit A, Homke R, Keller P . Diagnostic Molecular Mycobacteriology in Regions With Low Tuberculosis Endemicity: Combining Real-time PCR Assays for Detection of Multiple Mycobacterial Pathogens With Line Probe Assays for Identification of Resistance Mutations. EBioMedicine. 2016; 9:228-237. PMC: 4972562. DOI: 10.1016/j.ebiom.2016.06.016. View

Ben Selma W, Harizi H, Marzouk M, Ben Kahla I, Ben Lazreg F, Ferjeni A . Rapid detection of immunoglobulin G against Mycobacterium tuberculosis antigens by two commercial ELISA kits. Int J Tuberc Lung Dis. 2010; 14(7):841-6. View

Alavi-Naini R, Hashemi M, Mohagegh-Montazeri M, Sharifi-Mood B, Naderi M . Glutaraldehyde test for rapid diagnosis of pulmonary tuberculosis. Int J Tuberc Lung Dis. 2009; 13(5):601-5. View

Mase S, Ramsay A, Ng V, Henry M, Hopewell P, Cunningham J . Yield of serial sputum specimen examinations in the diagnosis of pulmonary tuberculosis: a systematic review. Int J Tuberc Lung Dis. 2007; 11(5):485-95. View

Khan M, Dar O, Sismanidis C, Shah K, Godfrey-Faussett P . Improvement of tuberculosis case detection and reduction of discrepancies between men and women by simple sputum-submission instructions: a pragmatic randomised controlled trial. Lancet. 2007; 369(9577):1955-60. DOI: 10.1016/S0140-6736(07)60916-7. View

De Kantor I, Lopez B, Torres P, Nader A, Garcia V . Preliminary evaluation of a simple method for detection of bovine tuberculosis: the glutaraldehyde test. Zentralbl Veterinarmed B. 1993; 40(1):27-30. DOI: 10.1111/j.1439-0450.1993.tb00105.x. View

10.

Marzouk M, Ferjani A, Dhaou M, Ali M, Hannachi N, Boukadida J . Comparison of LED and conventional fluorescence microscopy for detection of acid-fast bacilli in an area with high tuberculosis incidence. Diagn Microbiol Infect Dis. 2013; 76(3):306-8. DOI: 10.1016/j.diagmicrobio.2013.03.023. View

11.

Huang F, Dang L, Sun H, Yang H, Wu X . [A study of the value of three molecular diagnostic techniques in the diagnosis of tuberculosis]. Zhonghua Jie He He Hu Xi Za Zhi. 2015; 38(9):680-5. View

12.

Robson S, White N, Aronson I, Woollgar R, Goodman H, Jacobs P . Acute-phase response and the hypercoagulable state in pulmonary tuberculosis. Br J Haematol. 1996; 93(4):943-9. DOI: 10.1046/j.1365-2141.1996.d01-1722.x. View

13.

Bonnet M, Gagnidze L, Githui W, Guerin P, Bonte L, Varaine F . Performance of LED-based fluorescence microscopy to diagnose tuberculosis in a peripheral health centre in Nairobi. PLoS One. 2011; 6(2):e17214. PMC: 3041793. DOI: 10.1371/journal.pone.0017214. View

14.

Ben-Selma W, Ben-Kahla I, Marzouk M, Ferjeni A, Ghezal S, Ben-Said M . Rapid detection of Mycobacterium tuberculosis in sputum by Patho-TB kit in comparison with direct microscopy and culture. Diagn Microbiol Infect Dis. 2009; 65(3):232-5. DOI: 10.1016/j.diagmicrobio.2009.07.021. View

15.

Mathur M, Sachdev R . Temperature affects the results of the glutaraldehyde test in the diagnosis of pulmonary tuberculosis. Int J Tuberc Lung Dis. 2005; 9(2):200-5. View

16.

Minion J, Pai M, Ramsay A, Menzies D, Greenaway C . Comparison of LED and conventional fluorescence microscopy for detection of acid fast bacilli in a low-incidence setting. PLoS One. 2011; 6(7):e22495. PMC: 3141065. DOI: 10.1371/journal.pone.0022495. View

17.

Fu X, Ding M, Zhang N, Li J . Mycobacteriophages: an important tool for the diagnosis of Mycobacterium tuberculosis (review). Mol Med Rep. 2015; 12(1):13-9. DOI: 10.3892/mmr.2015.3440. View

18.

Steingart K, Henry M, Ng V, Hopewell P, Ramsay A, Cunningham J . Fluorescence versus conventional sputum smear microscopy for tuberculosis: a systematic review. Lancet Infect Dis. 2006; 6(9):570-81. DOI: 10.1016/S1473-3099(06)70578-3. View

19.

Larsson S, Shrestha M, Pokhrel B, Upadhyay M, Shrestha K . The glutaraldehyde test as a rapid screening method for pulmonary tuberculosis: a preliminary report. Ann Trop Med Parasitol. 1990; 84(2):111-7. DOI: 10.1080/00034983.1990.11812443. View

20.

Ben Kahla I, Ben Selma W, Marzouk M, Ferjeni A, Ghezal S, Boukadida J . Evaluation of a simplified IS6110 PCR for the rapid diagnosis of Mycobacterium tuberculosis in an area with high tuberculosis incidence. Pathol Biol (Paris). 2009; 59(3):161-5. DOI: 10.1016/j.patbio.2009.04.001. View