DNA-RNA Interactions Are Critical for Chromosome Condensation in

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2017 Nov 1

PMID 29087325

Citations 10

Authors

Zhong Qian

Victor B Zhurkin

Sankar Adhya

Affiliations

Soon will be listed here.

Abstract

Bacterial chromosome (nucleoid) conformation dictates faithful regulation of gene transcription. The conformation is condition-dependent and is guided by several nucleoid-associated proteins (NAPs) and at least one nucleoid-associated noncoding RNA, naRNA4. Here we investigated the molecular mechanism of how naRNA4 and the major NAP, HU, acting together organize the chromosome structure by establishing multiple DNA-DNA contacts (DNA condensation). We demonstrate that naRNA4 uniquely acts by forming complexes that may not involve long stretches of DNA-RNA hybrid. Also, uncommonly, HU, a chromosome-associated protein that is essential in the DNA-RNA interactions, is not present in the final complex. Thus, HU plays a catalytic (chaperone) role in the naRNA4-mediated DNA condensation process.

Citing Articles

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