Phosphorylation of Aspergillus Fumigatus PkaR Impacts Growth and Cell Wall Integrity Through Novel Mechanisms

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 2017 Oct 26

PMID 29067690

Citations 5

Authors

Elliot K Shwab

Praveen R Juvvadi

Greg Waitt

Erik J Soderblom

Martin A Moseley

Nathan I Nicely

William J Steinbach

Affiliations

Soon will be listed here.

Abstract

Protein kinase A (PKA) signaling is essential for growth and virulence of the fungal pathogen Aspergillus fumigatus. Little is known concerning the regulation of this pathway in filamentous fungi. Employing liquid chromatography-tandem mass spectroscopy, we identified novel phosphorylation sites on the regulatory subunit PkaR, distinct from those previously identified in mammals and yeasts, and demonstrated the importance of two phosphorylation clusters for hyphal growth and cell wall-stress response. We also identified key differences in the regulation of PKA subcellular localization in A. fumigatus compared with other species. This is the first analysis of the phosphoregulation of a PKA regulatory subunit in a filamentous fungus and uncovers critical mechanistic differences between PKA regulation in filamentous fungi compared with mammals and yeast species, suggesting divergent targeting opportunities.

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