Dissemination and Characteristics of a Novel Plasmid-Encoded Carbapenem-Hydrolyzing Class D β-Lactamase, OXA-436, Found in Isolates from Four Patients at Six Different Hospitals in Denmark

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 2017 Oct 25

PMID 29061750

Citations 16

Authors

Orjan Samuelsen

Frank Hansen

Bettina Aasnaes

Henrik Hasman

Bjarte Aarmo Lund

Hanna-Kirsti S Leiros

Berit Lilje

Jessin Janice

Lotte Jakobsen

Pia Littauer

Lillian M Soes

Barbara J Holzknecht

Leif P Andersen

Marc Stegger

Paal S Andersen

Anette M Hammerum

Affiliations

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Abstract

The diversity of OXA-48-like carbapenemases is continually expanding. In this study, we describe the dissemination and characteristics of a novel carbapenem-hydrolyzing class D β-lactamase (CHDL) named OXA-436. In total, six OXA-436-producing isolates, including ( = 3), ( = 2), and ( = 1), were identified in four patients in the period between September 2013 and April 2015. All three species of OXA-436-producing were found in one patient. The amino acid sequence of OXA-436 showed 90.4 to 92.8% identity to the amino acid sequences of other acquired OXA-48-like variants. Expression of OXA-436 in and kinetic analysis of purified OXA-436 revealed an activity profile similar to that of OXA-48 and OXA-181, with activity against penicillins, including temocillin; limited or no activity against extended-spectrum cephalosporins; and activity against carbapenems. The gene was located on a conjugative ∼314-kb IncHI2/IncHI2A plasmid belonging to plasmid multilocus sequence typing sequence type 1 in a region surrounded by chromosomal genes previously identified to be adjacent to genes in spp. In conclusion, OXA-436 is a novel CHDL with functional properties similar to those of OXA-48-like CHDLs. The described geographical spread among different and the plasmid location of illustrate its potential for further dissemination.

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