Porphyromonas Gingivalis Lipopolysaccharide Stimulation in Human Periodontal Ligament Stem Cells: Role of Epigenetic Modifications to the Inflammation

Overview

Journal Eur J Histochem

Specialty Biochemistry

Date 2017 Oct 20

PMID 29046054

Citations 33

Authors

Francesca Diomede

Soundara Rajan Thangavelu

Ilaria Merciaro

Monica DOrazio

Placido Bramanti

Emanuela Mazzon

Oriana Trubiani

Affiliations

Soon will be listed here.

Abstract

Periodontitis is a chronic oral inflammatory disease produced by bacteria. Gingival retraction and bone and connective tissues resorption are the hallmarks of this disease. Chronic periodontitis may contribute to the risk of onset or progression of neuroinflammatory pathological conditions, such as Alzheimer's disease. The main goal of the present study was to investigate if the role of epigenetic modulations is involved in periodontitis using human periodontal ligament stem cells (hPDLSCs) as an in vitro model system. hPDLSCs were treated with lipopolysaccharide of Porphyromonas gingivalis and the expression of proteins associated with DNA methylation and histone acetylation, such as DNMT1 and p300, respectively, and inflammatory transcription factor NF-kB, were examined. Immunofluorescence, Western blot and next generation sequencing results demonstrated that P. gingivalis lipopolysaccharide significantly reduced DNA methylase DNMT1, while it markedly upregulated the level of histone acetyltransferase p300 and NF-kB in hPDLSCs. Our results showed that P. gingivalis lipopolysaccharide markedly regulate the genes involved in epigenetic mechanism, which may result in inflammation induction. We propose that P. gingivalis lipopolysaccharide-treated hPDLSCs could be a potential in vitro model system to study epigenetics modulations associated with periodontitis, which might be helpful to identify novel biomarkers linked to this oral inflammatory disease.

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References

Bobetsis Y, Barros S, Lin D, Weidman J, Dolinoy D, Jirtle R . Bacterial infection promotes DNA hypermethylation. J Dent Res. 2007; 86(2):169-74. DOI: 10.1177/154405910708600212. View

Ogryzko V, Schiltz R, Russanova V, Howard B, Nakatani Y . The transcriptional coactivators p300 and CBP are histone acetyltransferases. Cell. 1996; 87(5):953-9. DOI: 10.1016/s0092-8674(00)82001-2. View

Demmer R, Behle J, Wolf D, Handfield M, Kebschull M, Celenti R . Transcriptomes in healthy and diseased gingival tissues. J Periodontol. 2008; 79(11):2112-24. PMC: 2637651. DOI: 10.1902/jop.2008.080139. View

Ganesh P, Karthikeyan R, Muthukumaraswamy A, Anand J . A Potential Role of Periodontal Inflammation in Alzheimer's Disease: A Review. Oral Health Prev Dent. 2017; 15(1):7-12. DOI: 10.3290/j.ohpd.a37708. View

Rajan T, Scionti D, Diomede F, Grassi G, Pollastro F, Piattelli A . Gingival Stromal Cells as an In Vitro Model: Cannabidiol Modulates Genes Linked With Amyotrophic Lateral Sclerosis. J Cell Biochem. 2016; 118(4):819-828. DOI: 10.1002/jcb.25757. View

Ide M, Harris M, Stevens A, Sussams R, Hopkins V, Culliford D . Periodontitis and Cognitive Decline in Alzheimer's Disease. PLoS One. 2016; 11(3):e0151081. PMC: 4786266. DOI: 10.1371/journal.pone.0151081. View

Colot V, Rossignol J . Eukaryotic DNA methylation as an evolutionary device. Bioessays. 1999; 21(5):402-11. DOI: 10.1002/(SICI)1521-1878(199905)21:5<402::AID-BIES7>3.0.CO;2-B. View

Scannapieco F, Cantos A . Oral inflammation and infection, and chronic medical diseases: implications for the elderly. Periodontol 2000. 2016; 72(1):153-75. DOI: 10.1111/prd.12129. View

Kornman K . Mapping the pathogenesis of periodontitis: a new look. J Periodontol. 2008; 79(8 Suppl):1560-8. DOI: 10.1902/jop.2008.080213. View

10.

Zhang P, Liu J, Xu Q, Harber G, Feng X, Michalek S . TLR2-dependent modulation of osteoclastogenesis by Porphyromonas gingivalis through differential induction of NFATc1 and NF-kappaB. J Biol Chem. 2011; 286(27):24159-69. PMC: 3129197. DOI: 10.1074/jbc.M110.198085. View

11.

Verrucci M, Pancrazzi A, Aracil M, Martelli F, Guglielmelli P, Zingariello M . CXCR4-independent rescue of the myeloproliferative defect of the Gata1low myelofibrosis mouse model by Aplidin. J Cell Physiol. 2010; 225(2):490-9. PMC: 3780594. DOI: 10.1002/jcp.22228. View

12.

Rajan T, Giacoppo S, Trubiani O, Diomede F, Piattelli A, Bramanti P . Conditioned medium of periodontal ligament mesenchymal stem cells exert anti-inflammatory effects in lipopolysaccharide-activated mouse motoneurons. Exp Cell Res. 2016; 349(1):152-161. DOI: 10.1016/j.yexcr.2016.10.008. View

13.

Gomez R, Dutra W, Moreira P . Epigenetics and periodontal disease: future perspectives. Inflamm Res. 2009; 58(10):625-9. DOI: 10.1007/s00011-009-0041-7. View

14.

Merika M, Williams A, Chen G, Collins T, Thanos D . Recruitment of CBP/p300 by the IFN beta enhanceosome is required for synergistic activation of transcription. Mol Cell. 1998; 1(2):277-87. DOI: 10.1016/s1097-2765(00)80028-3. View

15.

Sochocka M, Sobczynski M, Sender-Janeczek A, Zwolinska K, Blachowicz O, Tomczyk T . Association between Periodontal Health Status and Cognitive Abilities. The Role of Cytokine Profile and Systemic Inflammation. Curr Alzheimer Res. 2017; 14(9):978-990. PMC: 5676025. DOI: 10.2174/1567205014666170316163340. View

16.

Fraga M, Ballestar E, Villar-Garea A, Boix-Chornet M, Espada J, Schotta G . Loss of acetylation at Lys16 and trimethylation at Lys20 of histone H4 is a common hallmark of human cancer. Nat Genet. 2005; 37(4):391-400. DOI: 10.1038/ng1531. View

17.

Morsczeck C, Gotz W, Schierholz J, Zeilhofer F, Kuhn U, Mohl C . Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth. Matrix Biol. 2005; 24(2):155-65. DOI: 10.1016/j.matbio.2004.12.004. View

18.

Kraus D, Winter J, Jepsen S, Jager A, Meyer R, Deschner J . Interactions of adiponectin and lipopolysaccharide from Porphyromonas gingivalis on human oral epithelial cells. PLoS One. 2012; 7(2):e30716. PMC: 3271106. DOI: 10.1371/journal.pone.0030716. View

19.

Trubiani O, Guarnieri S, Diomede F, Mariggio M, Merciaro I, Morabito C . Nuclear translocation of PKCα isoenzyme is involved in neurogenic commitment of human neural crest-derived periodontal ligament stem cells. Cell Signal. 2016; 28(11):1631-41. DOI: 10.1016/j.cellsig.2016.07.012. View

20.

Lee Y, Lee H, Hu C, Huang L, Chao S, Lin C . Periodontitis as a Modifiable Risk Factor for Dementia: A Nationwide Population-Based Cohort Study. J Am Geriatr Soc. 2016; 65(2):301-305. DOI: 10.1111/jgs.14449. View