Proteomics of Eosinophil Activation

Overview

Journal Front Med (Lausanne)

Specialty General Medicine

Date 2017 Oct 17

PMID 29034237

Citations 6

Authors

Deane F Mosher

Emily M Wilkerson

Keren B Turton

Alexander S Hebert

Joshua J Coon

Affiliations

Soon will be listed here.

Abstract

We recently identified and quantified >7,000 proteins in non-activated human peripheral blood eosinophils using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) and described phosphoproteomic changes that accompany acute activation of eosinophils by interleukin-5 (IL5) (1). These data comprise a treasure trove of information about eosinophils. We illustrate the power of label-free LC-MS/MS quantification by considering four examples: complexity of eosinophil STATs, contribution of immunoproteasome subunits to eosinophil proteasomes, complement of integrin subunits, and contribution of platelet proteins originating from platelet-eosinophil complexes to the overall proteome. We describe how isobaric labeling enables robust sample-to-sample comparisons and relate the 220 phosphosites that changed significantly upon treatment with IL5 to previous studies of eosinophil activation. Finally, we review previous attempts to leverage the power of mass spectrometry to discern differences between eosinophils of healthy subjects and those with eosinophil-associated conditions and point out features of label-free quantification and isobaric labeling that are important in planning future mass spectrometric studies.

Citing Articles

Siglec-8 Signals Through a Non-Canonical Pathway to Cause Human Eosinophil Death .

Carroll D, Cao Y, Bochner B, OSullivan J Front Immunol. 2021; 12:737988.

PMID: 34721399 PMC: 8549629. DOI: 10.3389/fimmu.2021.737988.

Pin1 Regulates IL-5 Induced Eosinophil Polarization and Migration.

Shen Z, Hu J, ONeal M, Malter J Cells. 2021; 10(2).

PMID: 33494375 PMC: 7910834. DOI: 10.3390/cells10020211.

Eosinophil cytolysis on Immunoglobulin G is associated with microtubule formation and suppression of rho-associated protein kinase signalling.

Esnault S, Leet J, Johansson M, Barretto K, Fichtinger P, Fogerty F Clin Exp Allergy. 2019; 50(2):198-212.

PMID: 31750580 PMC: 6994386. DOI: 10.1111/cea.13538.

Myeloid-derived growth factor is a resident endoplasmic reticulum protein.

Bortnov V, Annis D, Fogerty F, Barretto K, Turton K, Mosher D J Biol Chem. 2018; 293(34):13166-13175.

PMID: 29954947 PMC: 6109940. DOI: 10.1074/jbc.AC118.002052.

Reuse of public, genome-wide, murine eosinophil expression data for hypotheses development.

Grace J, Malik A, Reichman H, Munitz A, Barski A, Fulkerson P J Leukoc Biol. 2018; 104(1):185-193.

PMID: 29758095 PMC: 6013373. DOI: 10.1002/JLB.1MA1117-444R.

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