DNA Damage and Oxidative Stress Induced by Seizures Are Decreased by Anticonvulsant and Neuroprotective Effects of Lobeline, a Candidate to Treat Alcoholism

Overview

Journal Metab Brain Dis

Publisher Springer

Specialties Endocrinology
Neurology

Date 2017 Oct 17

PMID 29032429

Citations 5

Authors

Liana Dantas da Costa E Silva

Patricia Pereira

Gabriela Gregory Regner

Fernanda Briao Menezes Boaretto

Cleonice Hoffmann

Pricila Pfluger

Lucas Lima da Silva

Luiza Reinhardt Steffens

Ana Moira Moras

Dinara Jaqueline Moura

Jaqueline Nascimento Picada

Affiliations

Soon will be listed here.

Abstract

The alkaloid lobeline (Lob) has been studied due to its potential use in treatment of drug abuse. This study evaluates the possible anticonvulsant and neuroprotective activities of Lob to obtain new information on its properties that could confirm it as a candidate in the treatment of alcohol addiction. The anticonvulsant effect of Lob was evaluated using a pilocarpine-induced seizure model. In addition, possible neuroprotective effects were investigated measuring DNA damage using the comet assay, assessing free radical levels by dichlorofluorescein diacetate (DCF) oxidation, and measuring the antioxidant potential using the α, α-diphenyl-β-picrylhydrazyl (DPPH) scavenging assay, besides measuring superoxide dismutase (SOD) and catalase (CAT) enzyme activities in brain tissues. Lobeline increased the latency to the first seizure and decreased the percentage of seizures in a similar way as diazepam, used as control. DNA damage induced by Pil and hydrogen peroxide were decreased in hippocampus and cerebral cortex from mice treated with Lob. The levels of free radicals and CAT activity increased in cortex and hippocampus, respectively, in mice treated with Pil. Lobeline decreased CAT in hippocampus, leading to similar values as in the saline negative control. In conclusion, Lob has anticonvulsant and neuroprotective actions that may be mediated by antioxidant-like mechanisms, indicating its potential as candidate drug in alcoholism therapy.

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