Self-assembly of Model Proteins into Virus Capsids

Overview

Journal J Phys Condens Matter

Specialties Biophysics
Biotechnology

Date 2017 Oct 14

PMID 29027904

Citations 3

Authors

Karol Wolek

Marek Cieplak

Affiliations

Soon will be listed here.

Abstract

We consider self-assembly of proteins into a virus capsid by the methods of molecular dynamics. The capsid corresponds either to SPMV or CCMV and is studied with and without the RNA molecule inside. The proteins are flexible and described by the structure-based coarse-grained model augmented by electrostatic interactions. Previous studies of the capsid self-assembly involved solid objects of a supramolecular scale, e.g. corresponding to capsomeres, with engineered couplings and stochastic movements. In our approach, a single capsid is dissociated by an application of a high temperature for a variable period and then the system is cooled down to allow for self-assembly. The restoration of the capsid proceeds to various extent, depending on the nature of the dissociated state, but is rarely complete because some proteins depart too far unless the process takes place in a confined space.

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