Synthesis and Characterization of Collagen/PLGA Biodegradable Skin Scaffold Fibers

Overview

Journal Regen Biomater

Specialty Biomedical Engineering

Date 2017 Oct 14

PMID 29026645

Citations 28

Authors

Alireza Sadeghi-Avalshahr

Samira Nokhasteh

Amir Mahdi Molavi

Mohammad Khorsand-Ghayeni

Meysam Mahdavi-Shahri

Affiliations

Soon will be listed here.

Abstract

The aim of this study is to investigate the applicability of poly(lactic-co-glycolic acid) (PLGA)/collagen composite scaffold for skin tissue engineering. PLGA and collagen were dissolved in HFIP as a common solvent and fibrous scaffolds were prepared by electrospinning method. The scaffolds were characterized by scanning electron microscopy (SEM), FTIR spectroscopy, mercury porosimetry, tensile strength, biocompatibility assays and Biodegradation. Cytotoxicity and cell adhesion were tested for two cell line groups, human dermal fibroblast (HDF) and human keratinocyte (HaCat). SEM images showed appropriate cell adhesion to the scaffold for both cell lines. MTT assays indicated that the cell viability of HDF cells increased with time, but the number of HaCat cells decreased after 14 days. The ultimate tensile strength was suitable for skin substitute application, but its elongation at break was rather low. For successful clinical application of the PLGA/collagen scaffold, some properties especially mechanical strain needs to be improved.

Citing Articles

Potentially commercializable nerve guidance conduits for peripheral nerve injury: Past, present, and future.

Liu C, Sun M, Lin L, Luo Y, Peng L, Zhang J Mater Today Bio. 2025; 31:101503.

PMID: 40018056 PMC: 11867546. DOI: 10.1016/j.mtbio.2025.101503.

Antitubercular Activity of 7-Methyljuglone-Loaded Poly-(Lactide Co-Glycolide) Nanoparticles.

Diedericks B, Kok A, Mandiwana V, Gordhan B, Kana B, Sinha Ray S Pharmaceutics. 2024; 16(11).

PMID: 39598600 PMC: 11597334. DOI: 10.3390/pharmaceutics16111477.

Investigating the Immunomodulatory Effects of Antigenic PLGA Nanoparticles and Nutritional Synergy in .

Baburajan A, Bhat S, Narayanan S Bionanoscience. 2024; 14(4):4493-4505.

PMID: 39404703 PMC: 7616597. DOI: 10.1007/s12668-024-01330-2.

CuO Nanoparticles as Nanocarriers and Its Antibacterial Efficacy.

Torres-Ramos M, Martin-Camacho U, Sanchez-Burgos J, Ghotekar S, Gonzalez-Vargas O, Fellah M Pharmaceuticals (Basel). 2024; 17(9).

PMID: 39338289 PMC: 11605230. DOI: 10.3390/ph17091124.

Alpha-boswellic acid accelerates acute wound healing via NF-κB signaling pathway.

Dong F, Zheng L, Zhang X PLoS One. 2024; 19(9):e0308028.

PMID: 39226297 PMC: 11371135. DOI: 10.1371/journal.pone.0308028.

References

Matthews J, Wnek G, Simpson D, Bowlin G . Electrospinning of collagen nanofibers. Biomacromolecules. 2002; 3(2):232-8. DOI: 10.1021/bm015533u. View

Li W, Laurencin C, Caterson E, Tuan R, Ko F . Electrospun nanofibrous structure: a novel scaffold for tissue engineering. J Biomed Mater Res. 2002; 60(4):613-21. DOI: 10.1002/jbm.10167. View

Garric X, Moles J, Garreau H, Braud C, Guilhou J, Vert M . Growth of various cell types in the presence of lactic and glycolic acids: the adverse effect of glycolic acid released from PLAGA copolymer on keratinocyte proliferation. J Biomater Sci Polym Ed. 2003; 13(11):1189-201. DOI: 10.1163/156856202320892957. View

Weinstein G, BOUCEK R . Collagen and elastin of human dermis. J Invest Dermatol. 1960; 35:227-9. View

Katti D, Robinson K, Ko F, Laurencin C . Bioresorbable nanofiber-based systems for wound healing and drug delivery: optimization of fabrication parameters. J Biomed Mater Res B Appl Biomater. 2004; 70(2):286-96. DOI: 10.1002/jbm.b.30041. View

Chen G, Sato T, Ohgushi H, Ushida T, Tateishi T, Tanaka J . Culturing of skin fibroblasts in a thin PLGA-collagen hybrid mesh. Biomaterials. 2004; 26(15):2559-66. DOI: 10.1016/j.biomaterials.2004.07.034. View

Witte R, Kao W . Keratinocyte-fibroblast paracrine interaction: the effects of substrate and culture condition. Biomaterials. 2004; 26(17):3673-82. DOI: 10.1016/j.biomaterials.2004.09.054. View

Badami A, Kreke M, Thompson M, Riffle J, Goldstein A . Effect of fiber diameter on spreading, proliferation, and differentiation of osteoblastic cells on electrospun poly(lactic acid) substrates. Biomaterials. 2005; 27(4):596-606. DOI: 10.1016/j.biomaterials.2005.05.084. View

Rho K, Jeong L, Lee G, Seo B, Park Y, Hong S . Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing. Biomaterials. 2005; 27(8):1452-61. DOI: 10.1016/j.biomaterials.2005.08.004. View

10.

Wu L, Zhang J, Jing D, Ding J . "Wet-state" mechanical properties of three-dimensional polyester porous scaffolds. J Biomed Mater Res A. 2005; 76(2):264-71. DOI: 10.1002/jbm.a.30544. View

11.

Powell H, Supp D, Boyce S . Influence of electrospun collagen on wound contraction of engineered skin substitutes. Biomaterials. 2007; 29(7):834-43. DOI: 10.1016/j.biomaterials.2007.10.036. View

12.

Christopherson G, Song H, Mao H . The influence of fiber diameter of electrospun substrates on neural stem cell differentiation and proliferation. Biomaterials. 2008; 30(4):556-64. DOI: 10.1016/j.biomaterials.2008.10.004. View

13.

Dong Y, Liao S, Ngiam M, Chan C, Ramakrishna S . Degradation behaviors of electrospun resorbable polyester nanofibers. Tissue Eng Part B Rev. 2009; 15(3):333-51. DOI: 10.1089/ten.TEB.2008.0619. View

14.

Lowery J, Datta N, Rutledge G . Effect of fiber diameter, pore size and seeding method on growth of human dermal fibroblasts in electrospun poly(epsilon-caprolactone) fibrous mats. Biomaterials. 2009; 31(3):491-504. DOI: 10.1016/j.biomaterials.2009.09.072. View

15.

Parenteau N, Nolte C, Bilbo P, Rosenberg M, Wilkins L, Johnson E . Epidermis generated in vitro: practical considerations and applications. J Cell Biochem. 1991; 45(3):245-51. DOI: 10.1002/jcb.240450304. View

16.

Noriega S, Hasanova G, Schneider M, Larsen G, Subramanian A . Effect of fiber diameter on the spreading, proliferation and differentiation of chondrocytes on electrospun chitosan matrices. Cells Tissues Organs. 2011; 195(3):207-21. PMC: 3697793. DOI: 10.1159/000325144. View

17.

Fritz J, Phillips B, Conkling N, Fourman M, Melendez M, Bhatnagar D . Comparison of native porcine skin and a dermal substitute using tensiometry and digital image speckle correlation. Ann Plast Surg. 2012; 69(4):462-7. DOI: 10.1097/SAP.0b013e31824a43b0. View

18.

Fiorani A, Gualandi C, Panseri S, Montesi M, Marcacci M, Focarete M . Comparative performance of collagen nanofibers electrospun from different solvents and stabilized by different crosslinkers. J Mater Sci Mater Med. 2014; 25(10):2313-21. DOI: 10.1007/s10856-014-5196-2. View

19.

Pelipenko J, Kocbek P, Kristl J . Nanofiber diameter as a critical parameter affecting skin cell response. Eur J Pharm Sci. 2014; 66:29-35. DOI: 10.1016/j.ejps.2014.09.022. View

20.

Kwak S, Haider A, Gupta K, Kim S, Kang I . Micro/Nano Multilayered Scaffolds of PLGA and Collagen by Alternately Electrospinning for Bone Tissue Engineering. Nanoscale Res Lett. 2016; 11(1):323. PMC: 4932007. DOI: 10.1186/s11671-016-1532-4. View