Low Immunogenicity of Mouse Induced Pluripotent Stem Cell-derived Neural Stem/progenitor Cells

Overview

Journal Sci Rep

Specialty Science

Date 2017 Oct 13

PMID 29021610

Citations 15

Authors

Go Itakura

Masahiro Ozaki

Narihito Nagoshi

Soya Kawabata

Yuichiro Nishiyama

Keiko Sugai

Tsuyoshi Iida

Rei Kashiwagi

Toshiki Ookubo

Kaori Yastake

Kohei Matsubayashi

Jun Kohyama

Akio Iwanami

Morio Matsumoto

Masaya Nakamura

Hideyuki Okano

Affiliations

Soon will be listed here.

Abstract

Resolving the immunogenicity of cells derived from induced pluripotent stem cells (iPSCs) remains an important challenge for cell transplant strategies that use banked allogeneic cells. Thus, we evaluated the immunogenicity of mouse fetal neural stem/progenitor cells (fetus-NSPCs) and iPSC-derived neural stem/progenitor cells (iPSC-NSPCs) both in vitro and in vivo. Flow cytometry revealed the low expression of immunological surface antigens, and these cells survived in all mice when transplanted syngeneically into subcutaneous tissue and the spinal cord. In contrast, an allogeneic transplantation into subcutaneous tissue was rejected in all mice, and allogeneic cells transplanted into intact and injured spinal cords survived for 3 months in approximately 20% of mice. In addition, cell survival was increased after co-treatment with an immunosuppressive agent. Thus, the immunogenicity and post-transplantation immunological dynamics of iPSC-NSPCs resemble those of fetus-NSPCs.

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