The Angiogenic Potential of DPSCs and SCAPs in an Model of Dental Pulp Regeneration

Overview

Journal Stem Cells Int

Publisher Wiley

Specialty Cell Biology

Date 2017 Oct 12

PMID 29018483

Citations 45

Authors

Petra Hilkens

Annelies Bronckaers

Jessica Ratajczak

Pascal Gervois

Esther Wolfs

Ivo Lambrichts

Affiliations

Soon will be listed here.

Abstract

Adequate vascularization, a restricting factor for the survival of engineered tissues, is often promoted by the addition of stem cells or the appropriate angiogenic growth factors. In this study, human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAPs) were applied in an model of dental pulp regeneration in order to compare their regenerative potential and confirm their previously demonstrated paracrine angiogenic properties. 3D-printed hydroxyapatite scaffolds containing DPSCs and/or SCAPs were subcutaneously transplanted into immunocompromised mice. After twelve weeks, histological and ultrastructural analysis demonstrated the regeneration of vascularized pulp-like tissue as well as mineralized tissue formation in all stem cell constructs. Despite the secretion of vascular endothelial growth factor , the stem cell constructs did not display a higher vascularization rate in comparison to control conditions. Similar results were found after eight weeks, which suggests both osteogenic/odontogenic differentiation of the transplanted stem cells and the promotion of angiogenesis in this particular setting. In conclusion, this is the first study to demonstrate the successful formation of vascularized pulp-like tissue in 3D-printed scaffolds containing dental stem cells, emphasizing the promising role of this approach in dental tissue engineering.

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References

Wu H, Kang N, Wang Q, Dong P, Lv X, Cao Y . The Dose-Effect Relationship Between the Seeding Quantity of Human Marrow Mesenchymal Stem Cells and In Vivo Tissue-Engineered Bone Yield. Cell Transplant. 2014; 24(10):1957-68. DOI: 10.3727/096368914X685393. View

Tran-Hung L, Mathieu S, About I . Role of human pulp fibroblasts in angiogenesis. J Dent Res. 2006; 85(9):819-23. DOI: 10.1177/154405910608500908. View

Bakopoulou A, Kritis A, Andreadis D, Papachristou E, Leyhausen G, Koidis P . Angiogenic Potential and Secretome of Human Apical Papilla Mesenchymal Stem Cells in Various Stress Microenvironments. Stem Cells Dev. 2015; 24(21):2496-512. PMC: 4620528. DOI: 10.1089/scd.2015.0197. View

Lobo S, Glickman R, da Silva W, Arinzeh T, Kerkis I . Response of stem cells from different origins to biphasic calcium phosphate bioceramics. Cell Tissue Res. 2015; 361(2):477-95. PMC: 4529461. DOI: 10.1007/s00441-015-2116-9. View

Wei F, Song T, Ding G, Xu J, Liu Y, Liu D . Functional tooth restoration by allogeneic mesenchymal stem cell-based bio-root regeneration in swine. Stem Cells Dev. 2013; 22(12):1752-62. PMC: 3668499. DOI: 10.1089/scd.2012.0688. View

Cavalcanti B, Zeitlin B, Nor J . A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells. Dent Mater. 2012; 29(1):97-102. PMC: 3515741. DOI: 10.1016/j.dental.2012.08.002. View

Nakashima M, Iohara K, Sugiyama M . Human dental pulp stem cells with highly angiogenic and neurogenic potential for possible use in pulp regeneration. Cytokine Growth Factor Rev. 2009; 20(5-6):435-40. DOI: 10.1016/j.cytogfr.2009.10.012. View

Bronckaers A, Hilkens P, Fanton Y, Struys T, Gervois P, Politis C . Angiogenic properties of human dental pulp stem cells. PLoS One. 2013; 8(8):e71104. PMC: 3737205. DOI: 10.1371/journal.pone.0071104. View

Yuan C, Wang P, Zhu L, Dissanayaka W, Green D, Tong E . Coculture of stem cells from apical papilla and human umbilical vein endothelial cell under hypoxia increases the formation of three-dimensional vessel-like structures in vitro. Tissue Eng Part A. 2014; 21(5-6):1163-72. PMC: 4356259. DOI: 10.1089/ten.TEA.2014.0058. View

10.

Vanacker J, Viswanath A, De Berdt P, Everard A, Cani P, Bouzin C . Hypoxia modulates the differentiation potential of stem cells of the apical papilla. J Endod. 2014; 40(9):1410-8. DOI: 10.1016/j.joen.2014.04.008. View

11.

Zhang R, Cooper P, Smith G, Nor J, Smith A . Angiogenic activity of dentin matrix components. J Endod. 2010; 37(1):26-30. DOI: 10.1016/j.joen.2010.08.042. View

12.

Hilkens P, Fanton Y, Martens W, Gervois P, Struys T, Politis C . Pro-angiogenic impact of dental stem cells in vitro and in vivo. Stem Cell Res. 2014; 12(3):778-90. DOI: 10.1016/j.scr.2014.03.008. View

13.

Gronthos S, Mankani M, Brahim J, Robey P, Shi S . Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A. 2000; 97(25):13625-30. PMC: 17626. DOI: 10.1073/pnas.240309797. View

14.

Partridge R, Conlisk N, Davies J . In-lab three-dimensional printing: an inexpensive tool for experimentation and visualization for the field of organogenesis. Organogenesis. 2012; 8(1):22-7. PMC: 3399707. DOI: 10.4161/org.20173. View

15.

Zhang W, Walboomers X, van Osch G, van den Dolder J, Jansen J . Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. Tissue Eng Part A. 2008; 14(2):285-94. DOI: 10.1089/tea.2007.0146. View

16.

Rosa V, Zhang Z, Grande R, Nor J . Dental pulp tissue engineering in full-length human root canals. J Dent Res. 2013; 92(11):970-5. PMC: 3797540. DOI: 10.1177/0022034513505772. View

17.

Huang G, Al-Habib M, Gauthier P . Challenges of stem cell-based pulp and dentin regeneration: a clinical perspective. Endod Topics. 2013; 28(1):51-60. PMC: 3727299. DOI: 10.1111/etp.12035. View

18.

Chiu Y, Fang H, Hsu T, Lin C, Shie M . The Characteristics of Mineral Trioxide Aggregate/Polycaprolactone 3-dimensional Scaffold with Osteogenesis Properties for Tissue Regeneration. J Endod. 2017; 43(6):923-929. DOI: 10.1016/j.joen.2017.01.009. View

19.

Mannoor M, Jiang Z, James T, Kong Y, Malatesta K, Soboyejo W . 3D printed bionic ears. Nano Lett. 2013; 13(6):2634-9. PMC: 3925752. DOI: 10.1021/nl4007744. View

20.

Berendsen A, Olsen B . How vascular endothelial growth factor-A (VEGF) regulates differentiation of mesenchymal stem cells. J Histochem Cytochem. 2013; 62(2):103-8. PMC: 3902099. DOI: 10.1369/0022155413516347. View