PLPP/CIN Regulates Seizure Activity by the Differential Modulation of Calsenilin Binding to GluN1 and Kv4.2 in Mice

Overview

Journal Front Mol Neurosci

Specialty Molecular Biology

Date 2017 Oct 11

PMID 28993724

Citations 4

Authors

Ji-Eun Kim

Hye-Won Hyun

Su-Ji Min

Duk-Shin Lee

A Ran Jeon

Min Ju Kim

Tae-Cheon Kang

Affiliations

Soon will be listed here.

Abstract

Calsenilin (CSEN) binds to Kv4.2 (an A-type K channel) as well as -methyl-D-aspartate receptor (NMDAR), and modulates their activities. However, the regulatory mechanisms for CSEN-binding to Kv4.2 or NMDAR remain elusive. Here, we demonstrate the novel role of pyridoxal-5'-phosphate phosphatase/chronophin (PLPP/CIN), one of the cofilin-mediated F-actin regulators, in the CSEN binding to Kv4.2 or GluN1 (an NMDAR subunit). PLPP/CIN dephosphorylated CSEN in competition with casein kinase 1, independent of cofilin dephosphorylation. As compared to wild-type mice, PLPP/CIN transgenic (PLPP/CIN) mice showed the enhancement of Kv4.2-CSEN binding, but the reduction in CSEN-GluN1 binding. In addition, PLPP/CIN mice exhibited the higher intensity (severity), duration and progression of seizures, but the longer latency of seizure on-set in response to kainic acid. PLPP/CIN knockout mice reversed these phenomena. Therefore, we suggest that PLPP/CIN-mediated CSEN dephosphorylation may play an important role in the functional coupling of NMDAR and Kv4.2, which regulates the neuronal excitability.

Citing Articles

PLPP/CIN-mediated NF2 S10 dephosphorylation distinctly regulates kainate-induced seizure susceptibility and neuronal death through PAK1-NF-κB-COX-2-PTGES2 signaling pathway.

Kim J, Lee D, Kim T, Park H, Kim M, Kang T J Neuroinflammation. 2023; 20(1):99.

PMID: 37118736 PMC: 10141957. DOI: 10.1186/s12974-023-02788-9.

PLPP/CIN-mediated NF2-serine 10 dephosphorylation regulates F-actin stability and Mdm2 degradation in an activity-dependent manner.

Kim J, Lee D, Kim T, Park H, Kim M, Kang T Cell Death Dis. 2021; 12(1):37.

PMID: 33414453 PMC: 7791067. DOI: 10.1038/s41419-020-03325-9.

PLPP/CIN-mediated NEDD4-2 S448 dephosphorylation regulates neuronal excitability via GluA1 ubiquitination.

Kim J, Lee D, Kim M, Kang T Cell Death Dis. 2019; 10(8):545.

PMID: 31320629 PMC: 6639327. DOI: 10.1038/s41419-019-1781-0.

PDI Knockdown Inhibits Seizure Activity in Acute Seizure and Chronic Epilepsy Rat Models via -Nitrosylation-Independent Thiolation on NMDA Receptor.

Jeon A, Kim J Front Cell Neurosci. 2018; 12:438.

PMID: 30524244 PMC: 6261974. DOI: 10.3389/fncel.2018.00438.

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