Anti-biofilm Activities from Leaves Against

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2017 Sep 29

PMID 28955316

Citations 27

Authors

Yucui Liu

Yanjie Xu

Qiuhang Song

Fei Wang

Luguo Sun

Lei Liu

Xiaoguang Yang

Jingwen Yi

Yongli Bao

Haifeng Ma

Honglan Huang

Chunlei Yu

Yanxin Huang

Yin Wu

Yuxin Li

Affiliations

Soon will be listed here.

Abstract

has been reported as a primary cariogenic pathogen associated with dental caries. The bacteria can produce glucosyltransferases (Gtfs) to synthesize extracellular polysaccharides (EPSs) that are known as virulence factors for adherence and formation of biofilms. Therefore, an ideal inhibitor for dental caries is one that can inhibit planktonic bacteria growth and prevent biofilm formation. (L.), widely used as a folk medicine and tea beverage, has been reported to have a variety of bioactivities. The present study aimed to explore the effect of (L.) leaf extracts on the biofilm of . The (L.) leaf extracts showed inhibitory effects by decreasing viability of bacteria within the biofilm, as evidenced by the XTT assay, live/dead staining assay and LDH activity assay, and could decrease the adherence property of through inhibiting Gtfs to synthesize EPSs. In addition, the reduced quantity of EPSs and the inhibition of Gtfs were positively correlated with concentrations of test samples. Finally, the MTT assay showed that the extracts had no cytotoxicity against normal oral cells. In conclusion, the extracts and sub-extracts of leaves were found to be antimicrobial and could reduce EPS synthesis by inhibiting activities of Gtfs to prevent bacterial adhesion and biofilm formation. Therefore, leaves have potential to be developed as a drug to prevent and cure dental caries.

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References

Hanada N, Kuramitsu H . Isolation and characterization of the Streptococcus mutans gtfD gene, coding for primer-dependent soluble glucan synthesis. Infect Immun. 1989; 57(7):2079-85. PMC: 313844. DOI: 10.1128/iai.57.7.2079-2085.1989. View

Palomino J, Martin A, Camacho M, Guerra H, Swings J, Portaels F . Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis. Antimicrob Agents Chemother. 2002; 46(8):2720-2. PMC: 127336. DOI: 10.1128/AAC.46.8.2720-2722.2002. View

Ooshima T, Osaka Y, Sasaki H, Osawa K, Yasuda H, Matsumura M . Caries inhibitory activity of cacao bean husk extract in in-vitro and animal experiments. Arch Oral Biol. 2000; 45(8):639-45. DOI: 10.1016/s0003-9969(00)00042-x. View

Liu R, Memarzadeh K, Chang B, Zhang Y, Ma Z, Allaker R . Antibacterial effect of copper-bearing titanium alloy (Ti-Cu) against Streptococcus mutans and Porphyromonas gingivalis. Sci Rep. 2016; 6:29985. PMC: 4960589. DOI: 10.1038/srep29985. View

Leme A, Koo H, Bellato C, Bedi G, Cury J . The role of sucrose in cariogenic dental biofilm formation--new insight. J Dent Res. 2006; 85(10):878-87. PMC: 2257872. DOI: 10.1177/154405910608501002. View

Song J, Kim S, Chang K, Han S, Yi H, Jeon J . In vitro inhibitory effects of Polygonum cuspidatum on bacterial viability and virulence factors of Streptococcus mutans and Streptococcus sobrinus. Arch Oral Biol. 2006; 51(12):1131-40. DOI: 10.1016/j.archoralbio.2006.06.011. View

Marchisio O, Esposito M, Genovesi A . Salivary pH level and bacterial plaque evaluation in orthodontic patients treated with Recaldent products. Int J Dent Hyg. 2010; 8(3):232-6. DOI: 10.1111/j.1601-5037.2009.00374.x. View

Kopec L, Vacca-Smith A, Bowen W . Structural aspects of glucans formed in solution and on the surface of hydroxyapatite. Glycobiology. 1997; 7(7):929-34. DOI: 10.1093/glycob/7.7.929. View

Shikov A, Pozharitskaya O, Makarova M, Kovaleva M, Laakso I, Dorman H . Effect of Bergenia crassifolia L. extracts on weight gain and feeding behavior of rats with high-caloric diet-induced obesity. Phytomedicine. 2012; 19(14):1250-5. DOI: 10.1016/j.phymed.2012.09.019. View

10.

Xiao J, Klein M, Falsetta M, Lu B, Delahunty C, Yates 3rd J . The exopolysaccharide matrix modulates the interaction between 3D architecture and virulence of a mixed-species oral biofilm. PLoS Pathog. 2012; 8(4):e1002623. PMC: 3320608. DOI: 10.1371/journal.ppat.1002623. View

11.

Liu C, Worthington R, Melander C, Wu H . A new small molecule specifically inhibits the cariogenic bacterium Streptococcus mutans in multispecies biofilms. Antimicrob Agents Chemother. 2011; 55(6):2679-87. PMC: 3101470. DOI: 10.1128/AAC.01496-10. View

12.

Xu X, Zhou X, Wu C . Tea catechin epigallocatechin gallate inhibits Streptococcus mutans biofilm formation by suppressing gtf genes. Arch Oral Biol. 2011; 57(6):678-83. DOI: 10.1016/j.archoralbio.2011.10.021. View

13.

Huang L, Yu F, Sun X, Dong Y, Lin P, Yu H . Antibacterial activity of a modified unfilled resin containing a novel polymerizable quaternary ammonium salt MAE-HB. Sci Rep. 2016; 6:33858. PMC: 5034341. DOI: 10.1038/srep33858. View

14.

Waters M, Kundu S, Lin N, Lin-Gibson S . Microstructure and mechanical properties of in situ Streptococcus mutans biofilms. ACS Appl Mater Interfaces. 2013; 6(1):327-32. DOI: 10.1021/am404344h. View

15.

Zhang D, Ren L, Zhang Y, Xue N, Yang K, Zhong M . Antibacterial activity against Porphyromonas gingivalis and biological characteristics of antibacterial stainless steel. Colloids Surf B Biointerfaces. 2013; 105:51-7. DOI: 10.1016/j.colsurfb.2012.12.025. View

16.

Koo H, Xiao J, Klein M, Jeon J . Exopolysaccharides produced by Streptococcus mutans glucosyltransferases modulate the establishment of microcolonies within multispecies biofilms. J Bacteriol. 2010; 192(12):3024-32. PMC: 2901689. DOI: 10.1128/JB.01649-09. View

17.

Hwang G, Klein M, Koo H . Analysis of the mechanical stability and surface detachment of mature Streptococcus mutans biofilms by applying a range of external shear forces. Biofouling. 2014; 30(9):1079-91. DOI: 10.1080/08927014.2014.969249. View

18.

Chen L, Ren Z, Zhou X, Zeng J, Zou J, Li Y . Inhibition of Streptococcus mutans biofilm formation, extracellular polysaccharide production, and virulence by an oxazole derivative. Appl Microbiol Biotechnol. 2015; 100(2):857-67. DOI: 10.1007/s00253-015-7092-1. View

19.

Huang R, Li M, Gregory R . Effect of nicotine on growth and metabolism of Streptococcus mutans. Eur J Oral Sci. 2012; 120(4):319-25. DOI: 10.1111/j.1600-0722.2012.00971.x. View

20.

Bowen W, Koo H . Biology of Streptococcus mutans-derived glucosyltransferases: role in extracellular matrix formation of cariogenic biofilms. Caries Res. 2011; 45(1):69-86. PMC: 3068567. DOI: 10.1159/000324598. View