MiR-543 Promotes Colorectal Cancer Proliferation and Metastasis by Targeting

Overview

Journal Oncotarget

Specialty Oncology

Date 2017 Sep 24

PMID 28938633

Citations 20

Authors

Fangbing Zhai

Chunhong Cao

Liang Zhang

Jianhua Zhang

Affiliations

Soon will be listed here.

Abstract

Till now, miR-543 expression has been demonstrated to be involved in the development of some cancers. However, reports about its expression and mechanism in colorectal cancer (CRC) were conflicting [1, 2]. Here, we investigated clinical implications of miR-543 and mechanisms underlying miR-543-mediated CRC development. In this study, real-time quantitative PCR (qRT-PCR) validated miR-543 was highly expressed in CRC samples and cell lines. MiR-543 was closely associated with tumor size, TNM stage and metastasis. In addition, survival analysis showed that high miR-543 expression was obviously correlated with poor overall survival and disease-free survival. Mechanically, downregulation of miR-543 by miR-543 inhibitor obviously repressed cell proliferation, promoted apoptosis, affected migration and invasion. Moreover, luciferase reporter analysis identified that Krüppel-like Factor-4 () was a direct target of miR-543, and there was an obvious inverse correlation between miR-543 and expression in CRC tissues. Furthermore, down-regulation favors miR-543-induced oncogenic effect on cell proliferation, apoptosis, migration, and invasion. In conclusion, this study indicated that miR-543 facilitates colorectal cancer proliferation and metastasis by targeting , and miR-543 may serve as a promising target for the treatment of CRC patients.

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