A Comprehensive, Cell Specific MicroRNA Catalogue of Human Peripheral Blood

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2017 Sep 22

PMID 28934507

Citations 100

Authors

Simonas Juzenas

Geetha Venkatesh

Matthias Hubenthal

Marc P Hoeppner

Zhipei Gracie Du

Maren Paulsen

Philip Rosenstiel

Philipp Senger

Martin Hofmann-Apitius

Andreas Keller

Limas Kupcinskas

Andre Franke

Georg Hemmrich-Stanisak

Affiliations

Soon will be listed here.

Abstract

With this study, we provide a comprehensive reference dataset of detailed miRNA expression profiles from seven types of human peripheral blood cells (NK cells, B lymphocytes, cytotoxic T lymphocytes, T helper cells, monocytes, neutrophils and erythrocytes), serum, exosomes and whole blood. The peripheral blood cells from buffy coats were typed and sorted using FACS/MACS. The overall dataset was generated from 450 small RNA libraries using high-throughput sequencing. By employing a comprehensive bioinformatics and statistical analysis, we show that 3' trimming modifications as well as composition of 3' added non-templated nucleotides are distributed in a lineage-specific manner-the closer the hematopoietic progenitors are, the higher their similarities in sequence variation of the 3' end. Furthermore, we define the blood cell-specific miRNA and isomiR expression patterns and identify novel cell type specific miRNA candidates. The study provides the most comprehensive contribution to date towards a complete miRNA catalogue of human peripheral blood, which can be used as a reference for future studies. The dataset has been deposited in GEO and also can be explored interactively following this link: http://134.245.63.235/ikmb-tools/bloodmiRs.

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