New Views on Phototransduction from Atomic Force Microscopy and Single Molecule Force Spectroscopy on Native Rods

Overview

Journal Sci Rep

Specialty Science

Date 2017 Sep 22

PMID 28931892

Citations 2

Authors

Sourav Maity

Nina Ilieva

Alessandro Laio

Vincent Torre

Monica Mazzolini

Affiliations

Soon will be listed here.

Abstract

By combining atomic force microscopy (AFM) imaging and single-molecule force spectroscopy (SMFS), we analyzed membrane proteins of the rod outer segments (OS). With this combined approach we were able to study the membrane proteins in their natural environment. In the plasma membrane we identified native cyclic nucleotide-gated (CNG) channels which are organized in single file strings. We also identified rhodopsin located both in the discs and in the plasma membrane. SMFS reveals strikingly different mechanical properties of rhodopsin unfolding in the two environments. Molecular dynamic simulations suggest that this difference is likely to be related to the higher hydrophobicity of the plasma membrane, due to the higher cholesterol concentration. This increases rhodopsin mechanical stability lowering the rate of transition towards its active form, hindering, in this manner, phototransduction.

Citing Articles

Unfolding and identification of membrane proteins in situ.

Galvanetto N, Ye Z, Marchesi A, Mortal S, Maity S, Laio A Elife. 2022; 11.

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Atomic force microscopy-single-molecule force spectroscopy unveils GPCR cell surface architecture.

Dague E, Pons V, Roland A, Azais J, Arcucci S, Lachaize V Commun Biol. 2022; 5(1):221.

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