The First Wave of T Lymphopoiesis in Zebrafish Arises from Aorta Endothelium Independent of Hematopoietic Stem Cells

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 2017 Sep 22

PMID 28931624

Citations 50

Authors

Ye Tian

Jin Xu

Shachuan Feng

Sicong He

Shizheng Zhao

Lu Zhu

Wan Jin

Yimei Dai

Lingfei Luo

Jianan Y Qu

Zilong Wen

Affiliations

Soon will be listed here.

Abstract

T lymphocytes are key cellular components of the adaptive immune system and play a central role in cell-mediated immunity in vertebrates. Despite their heterogeneities, it is believed that all different types of T lymphocytes are generated exclusively via the differentiation of hematopoietic stem cells (HSCs). Using temporal-spatial resolved fate-mapping analysis and time-lapse imaging, here we show that the ventral endothelium in the zebrafish aorta-gonad-mesonephros and posterior blood island, the hematopoietic tissues previously known to generate HSCs and erythromyeloid progenitors, respectively, gives rise to a transient wave of T lymphopoiesis independent of HSCs. This HSC-independent T lymphopoiesis occurs early and generates predominantly CD4 T cells in the larval but not juvenile and adult stages, whereas HSC-dependent T lymphopoiesis emerges late and produces various subtypes of T lymphocytes continuously from the larval stage to adulthood. Our study unveils the existence, origin, and ontogeny of HSC-independent T lymphopoiesis in vivo and reveals the complexity of the endothelial-hematopoietic transition of the aorta.

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