Effects of Aqueous Extract of Ruta Graveolens and Its Ingredients on Cytochrome P450, Uridine Diphosphate (UDP)-glucuronosyltransferase, and Reduced Nicotinamide Adenine Dinucleotide (phosphate) (NAD(P)H)-quinone Oxidoreductase in Mice

Overview

Journal J Food Drug Anal

Specialties Nutritional Sciences
Pharmacology
Toxicology

Date 2017 Sep 16

PMID 28911711

Citations 4

Authors

Yune-Fang Ueng

Chien-Chih Chen

Yu-Ling Huang

I-Jung Lee

Chul-Ho Yun

Yu-Hsuan Chen

Chiung-Chiao Huang

Affiliations

Soon will be listed here.

Abstract

Ruta graveolens (the common rue) has been used for various therapeutic purposes, including relief of rheumatism and treatment of circulatory disorder. To elucidate the effects of rue on main drug-metabolizing enzymes, effects of an aqueous extract of the aerial part of rue and its ingredients on cytochrome P450 (P450/CYP), uridine diphosphate (UDP)-glucuronosyltransferase, and reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H):quinone oxidoreductase were studied in C57BL/6JNarl mice. Oral administration of rue extract to males increased hepatic Cyp1a and Cyp2b activities in a dose-dependent manner. Under a 7-day treatment regimen, rue extract (0.5 g/kg) induced hepatic Cyp1a and Cyp2b activities and protein levels in males and females. This treatment increased hepatic UDP-glucuronosyltransferase activity only in males. However, NAD(P)H:quinone oxidoreductase activity remained unchanged. Based on the contents of rutin and furanocoumarins of mouse dose of rue extract, rutin increased hepatic Cyp1a activity and the mixture of furanocoumarins (F) increased Cyp2b activities in males. The mixture of rutin and F increased Cyp1a and Cyp2b activities. These results revealed that rutin and F contributed at least in part to the P450 induction by rue.

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