Macromolecular Profiling of Organelles in Normal Diploid and Cancer Cells

Overview

Journal Anal Chem

Specialty Chemistry

Date 2017 Sep 15

PMID 28910082

Citations 12

Authors

Svitlana M Levchenko

Andrey N Kuzmin

Artem Pliss

Junle Qu

Paras N Prasad

Affiliations

Soon will be listed here.

Abstract

To advance an understanding of cellular regulation and function it is crucial to identify molecular contents in cellular organelles, which accommodate specific biochemical processes. Toward achievement of this goal, we applied micro-Raman-Biomolecular Component Analysis assay for molecular profiling of major organelles in live cells. We used this assay for comparative analysis of proteins 3D conformation and quantification of proteins, RNA, and lipids concentrations in nucleoli, endoplasmic reticulum, and mitochondria of WI 38 diploid lung fibroblasts and HeLa cancer cells. Obtained data show substantial differences in the concentrations and conformations of proteins in the studied organelles. Moreover, differences in the intraorganellar concentrations of RNA and lipids between these cell lines were found. We report the biological significance of obtained macromolecular profiles and advocate for micro-Raman BCA assay as a valuable proteomics tool.

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