MicroRNA-451-modulated HnRNP A1 Takes a Part in Granulocytic Differentiation Regulation and Acute Myeloid Leukemia

Overview

Journal Oncotarget

Specialty Oncology

Date 2017 Sep 15

PMID 28903433

Citations 7

Authors

Li Song

Hai-Shuang Lin

Jia-Nan Gong

Hua Han

Xiao-Shuang Wang

Rui Su

Ming-Tai Chen

Chao Shen

Yan-Ni Ma

Jia Yu

Jun-Wu Zhang

Affiliations

Soon will be listed here.

Abstract

Myelopoiesis is under the control of a complex network containing various regulation factors. Deregulation of any important regulation factors may result in serious consequences including acute myeloid leukemia (AML). In order to find out the genes that may take a part in AML development, we analyzed data from AML cDNA microarray (GSE2191) in the NCBI data pool and noticed that heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is abnormally over-expressed in AML patients. Then we investigated the function and mechanisms of hnRNP A1 in myeloid development. A gradually decreased hnRNP A1 expression was detected during granulocytic differentiation in ATRA-induced-NB4 and HL-60 cells and cytokines-induced hematopoietic stem and progenitor cells. By function-loss and winning experiments we demonstrated hnRNP A1's inhibition role via inhibiting expression of C/EBPα, a key regulator of granulocytic differentiation, in the granulocytic differentiation. During granulocytic differentiation the decrease of hnRNP A1 reduces inhibition on C/EBPα expression, and the increased C/EBPα promotes the differentiation. We also demonstrated that miR-451 promotes granulocytic differentiation via targeting to and down-regulating hnRNP A1, and hnRNP A1 positively regulates c-Myc expression. Summarily, our results revealed new function and mechanisms of hnRNP A1 in normal granulocytiesis and the involvement of a feed-back loop comprising c-Myc, miR-451 and hnRNP A1 in AML development.

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References

Lewis S, Veyrier A, Ungureanu N, Bonnal S, Vagner S, Holcik M . Subcellular relocalization of a trans-acting factor regulates XIAP IRES-dependent translation. Mol Biol Cell. 2007; 18(4):1302-11. PMC: 1838995. DOI: 10.1091/mbc.e06-06-0515. View

Loh T, Moon H, Cho S, Jang H, Liu Y, Tai H . CD44 alternative splicing and hnRNP A1 expression are associated with the metastasis of breast cancer. Oncol Rep. 2015; 34(3):1231-8. DOI: 10.3892/or.2015.4110. View

Garzon R, Volinia S, Liu C, Fernandez-Cymering C, Palumbo T, Pichiorri F . MicroRNA signatures associated with cytogenetics and prognosis in acute myeloid leukemia. Blood. 2008; 111(6):3183-9. PMC: 2265455. DOI: 10.1182/blood-2007-07-098749. View

Marcucci G, Mrozek K, Radmacher M, Garzon R, Bloomfield C . The prognostic and functional role of microRNAs in acute myeloid leukemia. Blood. 2010; 117(4):1121-9. PMC: 3056468. DOI: 10.1182/blood-2010-09-191312. View

Jean-Philippe J, Paz S, Caputi M . hnRNP A1: the Swiss army knife of gene expression. Int J Mol Sci. 2013; 14(9):18999-9024. PMC: 3794818. DOI: 10.3390/ijms140918999. View

Douablin A, Deguillien M, Breig O, Baklouti F . HnRNP A1 tethers KSRP to an exon splicing silencer that inhibits an erythroid-specific splicing event in PU.1-induced erythroleukemia. Am J Cancer Res. 2015; 5(4):1410-22. PMC: 4473319. View

Chen H, Hewison M, Hu B, Adams J . Heterogeneous nuclear ribonucleoprotein (hnRNP) binding to hormone response elements: a cause of vitamin D resistance. Proc Natl Acad Sci U S A. 2003; 100(10):6109-14. PMC: 156334. DOI: 10.1073/pnas.1031395100. View

Nadiminty N, Tummala R, Liu C, Lou W, Evans C, Gao A . NF-κB2/p52:c-Myc:hnRNPA1 Pathway Regulates Expression of Androgen Receptor Splice Variants and Enzalutamide Sensitivity in Prostate Cancer. Mol Cancer Ther. 2015; 14(8):1884-95. PMC: 4529751. DOI: 10.1158/1535-7163.MCT-14-1057. View

Venables J . Unbalanced alternative splicing and its significance in cancer. Bioessays. 2006; 28(4):378-86. DOI: 10.1002/bies.20390. View

10.

Konishi H, Fujiya M, Ueno N, Moriichi K, Sasajima J, Ikuta K . microRNA-26a and -584 inhibit the colorectal cancer progression through inhibition of the binding of hnRNP A1-CDK6 mRNA. Biochem Biophys Res Commun. 2015; 467(4):847-52. DOI: 10.1016/j.bbrc.2015.10.055. View

11.

Takimoto M, Tomonaga T, Matunis M, Avigan M, Krutzsch H, Dreyfuss G . Specific binding of heterogeneous ribonucleoprotein particle protein K to the human c-myc promoter, in vitro. J Biol Chem. 1993; 268(24):18249-58. View

12.

Hamilton B, Burns C, Nichols R, Rigby W . Modulation of AUUUA response element binding by heterogeneous nuclear ribonucleoprotein A1 in human T lymphocytes. The roles of cytoplasmic location, transcription, and phosphorylation. J Biol Chem. 1997; 272(45):28732-41. DOI: 10.1074/jbc.272.45.28732. View

13.

Henics T, Sanfridson A, Hamilton B, Nagy E, Rigby W . Enhanced stability of interleukin-2 mRNA in MLA 144 cells. Possible role of cytoplasmic AU-rich sequence-binding proteins. J Biol Chem. 1994; 269(7):5377-83. View

14.

Wang X, Gong J, Yu J, Wang F, Zhang X, Yin X . MicroRNA-29a and microRNA-142-3p are regulators of myeloid differentiation and acute myeloid leukemia. Blood. 2012; 119(21):4992-5004. DOI: 10.1182/blood-2011-10-385716. View

15.

Jiang Z, Zhang W, Rao Y, Wu J . Regulation of Ich-1 pre-mRNA alternative splicing and apoptosis by mammalian splicing factors. Proc Natl Acad Sci U S A. 1998; 95(16):9155-60. PMC: 21308. DOI: 10.1073/pnas.95.16.9155. View

16.

Zheng D, Worthington J, Timms J, Woo P . HNRNPA1 interacts with a 5'-flanking distal element of interleukin-6 and upregulates its basal transcription. Genes Immun. 2013; 14(8):479-86. PMC: 3855448. DOI: 10.1038/gene.2013.41. View

17.

Wang F, Fu X, Chen P, Wu P, Fan X, Li N . SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling. Cell Res. 2017; 27(4):540-558. PMC: 5385621. DOI: 10.1038/cr.2017.7. View

18.

Yagi T, Morimoto A, Eguchi M, Hibi S, Sako M, Ishii E . Identification of a gene expression signature associated with pediatric AML prognosis. Blood. 2003; 102(5):1849-56. DOI: 10.1182/blood-2003-02-0578. View

19.

Metcalf D . Hematopoietic cytokines. Blood. 2008; 111(2):485-91. PMC: 2200848. DOI: 10.1182/blood-2007-03-079681. View

20.

Friedman A . C/EBPα in normal and malignant myelopoiesis. Int J Hematol. 2015; 101(4):330-41. PMC: 4696001. DOI: 10.1007/s12185-015-1764-6. View